Survivin在人肾透明细胞癌中的表达及其临床意义

Expression of inhibitor of apoptosis protein survivin in clear cell renal cell carcinoma and it's clinical significance

目的探讨凋亡抑制因子survivin蛋白在人肾透明细胞癌中的表达及其临床意义。方法采用免疫组织化学方法检测48例肾透明细胞癌组织和20例正常肾组织中survivin蛋白的表达及定位情况;并应用Western blot检测10例肾透明细胞癌组织及正常对照组织survivin蛋白的表达情况。结果Survivin蛋白在肾癌组织中表达,阳性率为89.58%(43/48),而正常组织中未见表达,二者有统计学差异(P<0.05);肾透明细胞癌中survivin蛋白的表达与患者有无远处转移、肾癌的Fuhrman分级及Robson分期呈正相关(P<0.05),而与肾癌患者的年龄、性别、肿瘤大小及有无下腔静脉癌栓无显著相关性。Western blot结果则进一步提示survivin高表达于肾透明细胞癌组织内,而不表达或极低表达于正常肾脏组织中。结论Survivin的高表达与肾透明细胞癌的分期、分级及进展具有密切相关性,提示了survivin在肾透明细胞癌的发生发展过程中可能扮演重要角色。

Objective To assess the expression of survivin, a member of the inhibitor of apoptosis protein family, in clear cell renal cell carcinoma （ccRCC） and to determine its clinical significance. Methods Immunohistochemical staining for survivin was performed using paraffin-embedded tissues from 48 cases of ccRCC and 20 cases of normal kidney tissues. We also assessed the expression of survivin in 10 cases of ccRCC by Western blot. Results Of the 48 ccRCC specimens, 43 （89.58%） showed positive staining of survivin immunohistochemically. In contrast, no expression of survivin was detected in nomal kidney tissues. A significant increase in survivin expression was associated with increased tumor stage, increased tumor grade and distant metastasis （P〈 0. 05）. However, there was no significant correlation between the expression of survivin and patient age, sex, tumor size and cancer embolus in inferior vena cava. Western blot analysis in 10 cases of ccRCC also demonstrated the high expression of survivin. Conclusion progression of renal cell carcinoma. This may be relevant for Survivin-mediated inhibition of apoptosis is associated with prognosis and alternative therapeutic approaches of ccRCC.