卡介苗诱导膀胱癌细胞cyclinD1、Fas表达的实验研究

Bacillus Calmette-Guerin inhibition the growth of bladder cancer cells through regulation of cyclinD_1 and Fas

目的观察卡介苗(BCG)对体外培养膀胱癌细胞生长情况及相关基因(cyclinD1、Fas)表达的影响,并探讨其可能的作用机制。方法MTT法检测不同浓度BCG(0.15、0.3、0.6、1.2mg/mL)对人膀胱癌BIU-87细胞生长的影响。流式细胞术(FCM)检测BCG处理后膀胱癌细胞周期分布的变化及对膀胱癌细胞凋亡的影响;逆转录-聚合酶链反应(RT-PCR)检测BCG作用BIU-87细胞后cyclinD1、FasmRNA表达的变化。结果BCG体外能明显抑制BIU-87细胞的生长,且抑制作用呈明显的剂量、时间依赖性(P<0.01)。流式细胞术检测示处理后膀胱癌细胞阻滞于G0～G1期,RT-PCR结果示BCG处理能显著诱导FasmRNA的表达和显著抑制cyclinD1mRNA的表达(P<0.05)。结论TSA可通过诱导细胞凋亡和使细胞阻滞于G1期而发挥体外抗膀胱癌作用,其作用机制可能涉及相关基因(cyclinD1、Fas)表达的调控。

Objective To investigate the influence of Bacillus Calmette-Guerin （BCG） on the growth of human bladder cancer cells and the expression of related genes, and to explore the involved mechanism. Methods A human bladder cancer cell line, BIU-87, was treated with different concentrations（0.15, 0.3, 0.6, 1.2 mg/mL） of BCG. After treatment, cell growth was measured by MTT assay. Cell apoptosis changes and the cell cycle distribution were examined by flow cytometry （FCM）.mRNA expression of cyclinD1 and Fas was assessed by reverse transcription-polymerase chain reaction. Results Bacillus Calmette-Guerin significantly inhibited the proliferation of bladder cancer cell in a time- and dose-dependent manner. Flow cytometry showed that the cells were blocked at G0-G1 phase and cell apoptosis increased. It was showed by reverse transcription-polymerase chain reaction that the Fas mRNA level increased while the cyclinD1 mRNA level dropped after BCG treatment. Oonclusion The results indicate that BCG is able to inhibit bladder cancer cell growth in vitro by inducing cell apoptosis and cell cycle arrest,which might be related to the expression of cyclinD1 and Fas.