角膜移植后角膜新生淋巴管与新生血管间的关联(英文)

Corneal lymphangiogenesis correlates closely with hemangiogenesis after keratoplasty

目的:研究角膜移植后角膜新生淋巴管与新生血管间关联。方法:人角膜取自行二次角膜移植的19名患者。5核苷酸酶-碱性磷酸酶(5-nase-Alkaline phosphatase,5-NA-ALP)双重酶组织化学染色及淋巴内皮细胞受体(lymphatic vessel endothelial receptor,LYVE-1)、内皮细胞黏附因子-1(platelet endothelial cell adhesion modecule-1,PECAM-1)双重免疫组化法标记角膜中的新生血管和淋巴管,并进行淋巴管计数(lymphatic vessels counting,LVC)和血管计数(blood vessels counting,BVC),比较BVC与LVC之间的关联。结果:角膜中存在角膜新生血管12例(63%),存在角膜新生淋巴管5例(26%)。角膜新生淋巴管仅出现在血管化角膜中。角膜移植后BVC与LVC间呈显著性正相关(r=0.725;P<0.01)。结论:人角膜移植后角膜新生淋巴管与新生血管之间存在密切关联。

AIM： To examine the relationship between corneal lymphangiogenesis and hemangiogenesis after keratoplasty. METHODS： Nineteen human corneas were obtained from 19 patients undergoing a second corneal transplantation in Zhongshan Ophthalmic Center in 2005. Blood and lymphatic vessels in human transplanted corneas were identified by lymphatic vessel endothelial receptor（LYVE- 1 ） and platelet endothelial cell adhesion modecule-1 （PECAM-1） immunohistochemistry, and double enzymehistochemistry; then the association of corneal blood vessel counting （BVC） with lymphatic vessel counting （LVC） was examined. RESULTS： Corneal hemangiogenesis was present in 12 cases （63%）, and lymphangiogenesis occurred in 5 cases （26%） of human transplanted corneas. In addition, corneal lymphangiogenesis was only present in vascularized corneas. LVC was strongly and positively correlated with BVC（ r = 0. 725 ; P〈 0.01 ）. CONCLUSION： Corneal lymphangiogenesis develops after keratoplasty, and strongly associates with hemangiogenesis.