视网膜色素上皮细胞诱导骨髓间充质干细胞分化为神经样细胞(英文)

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigment epithelial cells

目的:检测视网膜色素上皮细胞对骨髓间充质干细胞分化的调节作用。方法:体外培养人骨髓间充质干细胞(BMSC)和视网膜色素上皮(RPE)细胞。RPE细胞采用紫外线照射处理,而后与CFSE标记的BMSC共培养14d。并在共培养体系中加入牛眼视网膜提取物(BRE)以研究视网膜成分对此分化过程的影响。采用NSE,Nestin和GFAP抗体标记检测BMSC分化前后的表达特征。结果:BMSC在与RPE细胞共培养后,能够分化成为神经样细胞,并表达神经性细胞的特异性标记NSE,Nestin和GFAP。BRE能够显著促进共培养体系中BMSC向神经样细胞的分化。结论:RPE和BRE能够诱导BMSC分化为神经样细胞。

AIM： To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells （BMSC）. METHODS： Human fetal BMSC were previously labeled by carboxyfluorescein succinimidyl ester （CFSE）, and cocultured with retinal pigment epithelial （RPE） cells which were pre-treated with ultraviolet irradiation at a ratio of 1：1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract （BRE） was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, neuron-specific enolase （NSE）, Nestin and Glial fibrillary acidic protein （GFAP） immunostaining were performed to determine the characteristics of BMSC. RESULTS： The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION： Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.