川芎嗪对大鼠离体培养晶状体SOD,CAT和MDA的影响

Effect of tetramethylpyrazine on SOD,CAT and MDA of isolated rat lens

目的:探索川芎嗪(tetramethylpyrazine,TMP)对大鼠离体培养晶状体抗氧化作用的SOD,CAT和MDA的影响。方法:取SD大鼠晶状体放入0,0.125,0.25,0.5,1,2,4g/L的TMP溶液的培养液进行离体培养24h后,观察晶状体形态变化,并作组织匀浆后采用黄嘌呤氧化酶反应法测定SOD活性,钼酸铵终止法测定CAT活性,硫代巴比妥酸法测定MDA含量。结果:与正常对照组比较,0.25,0.5,1和2g/L的TMP可明显提高大鼠晶状体中SOD的活性(P<0.01,P<0.01,P<0.01,P<0.05),而0.125和4g/L的TMP作用效果不明显。CAT检测结果显示,与正常对照组比较,0.5g/L的TMP可明显提高大鼠晶状体中CAT的活性(P<0.01),而0.125,0.25,1,2和4g/L的TMP作用效果不明显。与正常对照组比较,0.125,0.25,0.5,1和2g/L的TMP可明显降低大鼠晶状体中MDA的含量(P<0.01),4g/L的TMP作用效果不明显。结论:TMP对培养大鼠离体晶状体有提高SOD,CAT活性和降低MDA含量的作用。

AIM： To observe the effect of tetramethylpyrazine （TMP） on superoxide dismutase （ SOD）, malonaldehyde （MDA） and catalase （CAT） of isolated rat lens in vitro . METHODS： The rats were randomly divided into 7 groups and the lens in each group were isolated and cultured with RPMI-1640 culture fluid and different concentrations of TMP solution （0,0.125, 0.25, 0.5, 1, 2 and 4g/L）. After cultured for 24 hours, the transparency of the lens was observed with wake eye and homogenated. The enzyme activation levels of SOD,CAT and the contents of MDA were measured by photometry respectively. RESULTS： Compared with normal group, 0.25, 0.5, 1 and 2g/L TMP could obviously increase the activity of SOD, P 〈 0.01, P 〈 0.01, P 〈 0.01 and P 〈 0.05 respectively, while 0. 125 and 4g/L TMP had no significance effect. Compared with normal group, 0.5g/L TMP could improve the activity of CAT, P〈 0.01 while other concentrations of TMP had no significance effect. Compared with normal group, 0.125, 0.25, 0.5, 1 and 2g/L TMP could obviously decrease the content of MDA, P 〈 0.01, while 4g/L TMP had no significance effect.CONCLUSION ： TMP could increase enzyme activation of SOD and CAT, and decrease contents of MDA in isolated lens of rats.