高糖对视网膜Müller细胞VEGF,EPO和EPOR mRNA表达的影响

Effect of high levels of glucose on expressions of VEGF,EPO and EPOR in retinal Müller cells

目的:观察高糖条件下VEGF mRNA,EPO mRNA和EPORmRNA在体外培养的Mller细胞中的表达情况。方法:胰蛋白酶将新生小鼠视网膜组织吹打消化后,制成单细胞悬液,体外培养Mller细胞,RT-PCR测定高糖条件下视网膜Mller细胞VEGF,EPO和EPOR基因的表达。结果:成功获得视网膜Mller细胞,传代后90%以上的细胞呈兔抗鼠谷氨酰胺合酶(GS)染色阳性。Mller细胞VEGF mRNA,EPO mRNA和EPOR mRNA在高糖条件下表达升高,且呈浓度依赖性(P<0.05),但糖浓度50mmol/L组较40mmol/L组差异无统计学意义,无明显时间依赖性。结论:Mller细胞在高糖条件下VEGF,EPO,EPOR的表达增加。

AIM ：To observe the expressions of vascular endothelial growth factor （VEGF）, erythropoietin （EPO）, erythropoietin receptor（ EPOR） mRNA in different levels of glucose in the retinal Muller cells of mice in vitro. METHODS： MUller cells of new-born mice were cultured with enzyme digestion. Muller cells were separated and purified by blowing and striking method. The expressions of VEGF mRNA, EPO mRNA, and EPOR mRNA were detected by RT-PCR. RESULTS： Retinal MUller cells were cultured successfully, 90% of which were positively stained by glutamine synthetase （GS）. The expressions of VEGF mRNA, EPO mRNA and EPOR mRNA in retinal Muller cells were significantly enhanced in the high glucose group which increased obviously（ P〈 0. 05）, but the difference between 50mmol/L group and the 40mmol/L group did not have statistical significance or time dependence. CONCLUSION： With glucose concentration elevating, VEGF, EPO, EPOR expression of cultured retinal Muller cells increased.