摘要
获取旋毛虫肌幼虫排泄分泌(ES)抗原的部分结构基因并进行了克隆、鉴定和表达。首先采用RTPCR技术从旋毛虫肌幼虫总RNA中获取目的基因,经酶切分析后与融合表达载体pEX31C、pEX31B及非融合表达载体pBV220连接,并将其转入大肠杆菌进行表达。结果:重组质粒在大肠杆菌中表达出相应的相对分子质量的重组蛋白,经纯化后的重组蛋白可与旋毛虫病猪阳性血清发生反应。
The partial structure gene encoding excretory secretory (ES) antigen derived from T.spiralis (TSP) muscle larvae was cloned,characterized and expressed in E.coli.The target gene was directly obtained by using RT PCR technique from total T.spiralis RNA.It was analyzed by RE digestion and was cloned into the expression vector pEX31C,pEX31B and infusion expression vector pBV220.The recombinant plasmids were transferred into E.coli to show their expression level and the specificity of the expression products was also identified.The results revealed that three recombinant plasmids expressed the corresponding proteins.All the recombinant proteins could be recognized by sera from swine infected with T.spiralis.
出处
《河南医科大学学报》
CAS
1998年第4期100-102,共3页
Journal of Henan Medical University
关键词
旋毛虫病
基因重组
ES抗原
排泄
分泌
Trischinella spiralis, gene cloning,RT PCR,excretory secretory (ES) antigen