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旋毛虫排泄-分泌抗原重组的研究 被引量:1

Clonging of High Level Expression of ES Antigen from Trichinella Spiralis Muscle larvae
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摘要 获取旋毛虫肌幼虫排泄分泌(ES)抗原的部分结构基因并进行了克隆、鉴定和表达。首先采用RTPCR技术从旋毛虫肌幼虫总RNA中获取目的基因,经酶切分析后与融合表达载体pEX31C、pEX31B及非融合表达载体pBV220连接,并将其转入大肠杆菌进行表达。结果:重组质粒在大肠杆菌中表达出相应的相对分子质量的重组蛋白,经纯化后的重组蛋白可与旋毛虫病猪阳性血清发生反应。 The partial structure gene encoding excretory secretory (ES) antigen derived from T.spiralis (TSP) muscle larvae was cloned,characterized and expressed in E.coli.The target gene was directly obtained by using RT PCR technique from total T.spiralis RNA.It was analyzed by RE digestion and was cloned into the expression vector pEX31C,pEX31B and infusion expression vector pBV220.The recombinant plasmids were transferred into E.coli to show their expression level and the specificity of the expression products was also identified.The results revealed that three recombinant plasmids expressed the corresponding proteins.All the recombinant proteins could be recognized by sera from swine infected with T.spiralis.
出处 《河南医科大学学报》 CAS 1998年第4期100-102,共3页 Journal of Henan Medical University
关键词 旋毛虫病 基因重组 ES抗原 排泄 分泌 Trischinella spiralis, gene cloning,RT PCR,excretory secretory (ES) antigen
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参考文献8

  • 1Gamble HR,Anderson WR,Graham CE,et al.Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay(ELISA)using an excretory-secretory antigen.Vet Parasitol,1983,13:349.
  • 2Su XZ,Prestwood AK A dot-ELISA mimicry Westerm blot test for the detection of swine trichinellosis.J Parasitol,1991,77:76.
  • 3许威光,袁文甫,王家祥,周光悌,马增全.旋毛虫肌幼虫排泄—分泌抗原诊断猪旋毛虫病的研究与应用[J].华北农学报,1989,4(4):133-139. 被引量:14
  • 4Chomczynski P,Sacchi N,Single-step method of RNA isolation by acid guanidinium thiocyante-phenol-cholroform extraction.Anal Biolchem,1987,162:156.
  • 5阎玉河,朱元晓,许威光,陈辉,马增全,王克领.旋毛虫肌幼虫RNA的提取及目的基因的PCR扩增[J].畜牧兽医学报,1994,25(3):262-267. 被引量:9
  • 6萨姆布鲁克J,弗里奇EF,曼尼阿蒂斯T,著(金东雁,等译).分子克隆实验指南.第2版.北京:科学出版社,1995.24-26
  • 7Strebel K,Beck E,Strohmaier K,et al.Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion protein.J virol,1986,57:983.
  • 8Sugane K,Matsuura T,Molecular analysis of the gene encoding an antigenic polypeptide of Trichinella spiralis infective larvae, J Helminthol,1990,64:1.

二级参考文献6

  • 1Su X,J Parasitol,1991年,77卷,76页
  • 2许威光,华北农学报,1989年,4卷,133页
  • 3王槐春,生物化学杂志,8卷,342页
  • 4张建安,付重林.ELISA诊断猪旋毛虫病条件的研究[J]中国兽医科技,1986(11).
  • 5王栋,陈宝贵,梁建明,朱钦文.酶联免疫吸附试验检测人工感染羊脑脊髓丝虫病抗体的研究[J]中国兽医科技,1985(05).
  • 6牛炳亨,潘光武,何惟,窦兰清.应用酶联免疫吸附试验诊断猪旋毛虫病的研究[J]中国兽医科技,1985(02).

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