TGF-βRⅡ在直肠癌中的表达及其临床意义

Clinical Significance of TGF βR Ⅱ Expression in Human Rectal Cancer

目的：研究ＴＧＦ－βＲⅡ表达与直肠癌生物学行为和预后的关系。方法：应用免疫组化Ｓ－Ｐ方法检测７８例直肠癌手术标本中ＴＧＦ－βＲⅡ的表达情况。结果：直肠癌原发灶ＴＧＦ－βＲⅡ表达阳性率为４８．７２％，其中无区域淋巴结转移者阳性率为８２．１４％，有区域性淋巴结转移者阳性率为３０．００％（Ｐ＜０．０１），ＴＧＦ－βＲⅡ表达水平与直肠癌浸润深度和Ｄｕｋｅｓ分期呈负相关（Ｐ＜０．０１，Ｐ＜０．０１）。ＴＧＦ－β阳性组术后３年、５年生存率分别为７８．０３％，６９．０２％，而阴性者为３３．８０％、１３．７５％，两组患者术后３年、５年生存率差别均有显著性意义（Ｐ＜０．０１，Ｐ＜０．０１）。结论：直肠癌由于ＴＧＦ－βＲⅡ减少而逃逸ＴＧＦ－β的生长抑制作用，加速恶性细胞的增殖、浸润和转移。故检测直肠癌组织中ＴＧＦ－βＲⅡ表达状况。

Objective: To study the relationship between TGF βR Ⅱ (transforming growth factor β receptor type Ⅱ) expression and biological behavior or prognosis of rectal cancer. Methods: The expression of TGF βR Ⅱ was examined immunohistochemically in resected rectal cancers using anti polyclonal antibody.Results: Of 78 patients tested, 48.72%(38 cases) showed positive staining of TGF βR Ⅱ in the primary lesions,while the others were negative. Tumors without regional node metastasis showed a significantly higher positive staining rate 82.14%(23/28) than those with node involvement 30.00%(15/50 )( P < 0.01 ). Inverse correlations were found between TGF B II expression and tumor invasion ( P <0.01) or the progression of Dukes stage ( P <0.01). Expression of TGF βR Ⅱ was positively associated with longer postoperative survival P <0.01). Conclusions: The data indicated that reduction of TGF βR Ⅱ resulted in escape from growth inhibitory effect of TGF B and accelerated the proliferation of malignant cells and its invasion and metastasis. The expression of TGF βR Ⅱ may be a useful marker in the assessment of the prognosis of rectal cancer.