环氧氯丙烷和Triton X-100联合处理猪主动脉瓣的实验研究

Experimental Study of Protective Effects of Epoxy Chloropropane and Triton X 100 against Calcification in Glutaraldehyde treated Procine Aortic Valve

用环氧氯丙烷（ＥＣ）和（或）ＴｒｉｔｏｎＸ１００分别对戊二醛（ＧＡ）处理的猪主动脉瓣进行化学改性，以单纯ＧＡ处理的猪瓣作对照。经新西兰幼兔皮下埋藏２、４、６、８周后分别应用原子吸收光谱、光镜及电镜观察，对钙质进行定量、定性和形态学研究。发现ＥＣ＋ＴｒｉｔｏｎＸ１００组的钙含量最低、组织形态学示钙化程度最轻，ＥＣ组和ＴｒｉｔｏｎＸ１００组次之，ＧＡ组最重。实验证明：ＥＣ＋ＴｒｉｔｏｎＸ１００联合化学改性ＧＡ处理的猪主动脉瓣能明显提高防钙化的效果。且能保持猪主动脉瓣完整的组织结构并提高猪主动脉瓣组织的稳定性。急性毒性试验证明，经ＥＣ处理和ＥＣ＋ＴｒｉｔｏｎＸ１００联合处理的猪主动脉瓣无毒性反应。

Aim:This experimental study was undertaken to evaluate the effects of epoxy chloropropane and Triton X 100 on calcification of glutaraldehyde treated procine aortic valve.Method:The glutaraldehyde(GA) treated porcine aortic valves were modified chemically by using epoxy chloropropane(EC),epoxy chloropropane plus Triton X 100 and Triton X 100 respectively.The conventionally prepared GA treated valves were used as a control group.The treated valves were implanted subcutaneously in New Zealand rabbits for 2、4、6 and 8 weeks.Quantitative,Qualitative and morphologic study of the calcium deposit of the implanted valves were made by atomic absorption,light microscope and electron microscopes.Result:It was found that the calcium content was the lowest in Ec+ Triton X 100 group and the highest in GA group.Histological observations also showed that the process of cation cafim was much delayed in the EC+Triton X 100 group.Conclusion:It was demonstrated that chemical modification of GA treated porcine aortic valves by EC+Triton X 100 could suppress valve calcification effectively and preserve its structure and improve its tissue stability.