钙泊三醇外用治疗可减少银屑病皮损中ICAM-3阳性浸润细胞的表达

Topical Treatment of Calcipotriol Decreases Expression of ICAM-3 Positive Infiltrating Leukocytes in Psoriatic Skin

目的了解钙泊三醇局部外用治疗对银屑病皮损中细胞间粘附分子3(ICAM-3)表达的影响。方法采用 ABC 免疫组化法对20例银屑病患者皮损与非皮损部位以及皮损部位治疗前后的ICAM-3、Ki-67和其它免疫分子的表达进行了研究。结果银屑病皮损部位 ICAM-3阳性浸润细胞的表达均明显高于非皮损部位及正常对照组(P 均<0.01),而且 ICAM-3的表达和皮损部位 Ki-67.CD3等免疫分子的表达呈正相关。经钙泊三醇外用治疗6周后、皮损部位 ICAM-3、Ki-67 和 CD3的表达均明显减少(P 均<0.01)。结论外用钙泊三醇治疗银屑病的机制除了有抗角朊细胞增殖的作用外,对可能参与银屑病发病机制的 ICAM-3阳性浸润细胞也有作用。

Objective To study the effeet of topical caleipotriol treatment on the expression ICAM-3 molecule in infiltrating leukoeyles of skin lesions of patients with psoriasis berore and after lrealmenl. Methods Expression of ICAM-3. Ki-67 and CD3 was studied in the skin biopsies from 20 palients with chronicn plaque type psoriasis before and after topeal ealeipotriol treatment by using an immnohistoehieal leehnique-Adin biotin Complex(ABC)System Results ICAM-3.Ki-67 and CD3 posilive slaining cells in the skin lesions were significanlly higher than those in uninvolved skin as well as those of normal controls.and there was a posilive conelation between the lesional expression of ICAM-3 and Ki-67 molecues,as well as between lesional expres- sion of ICAM-3 and CD3 molecules.After six weeks of topieal applieation with eaeopotriol the expression of I- CAM-3. Ki-67 and CD3 in involved skin were significant decreased(P<0.01).Conclusion It is indieated thai the.meehanism of topical tretment wiih caleipotriol in psoriasis involves not only only inbibiting keralinoeyte poliferation.but also effecting ICAM-3 infirating leukoeyvtes in lesional skin.which may be involved in the pathogenesis of psoriasis.