SPR缓解痉挛型脑瘫泌涎症状的动物实验研究

Experiment of Selective Posterior Rhizotomy Remitting Salivation in Spastic Cerebral Palsy

目的研究选择性脊神经后根切断术(SPR)缓解痉挛型脑瘫动物泌涎症状的机理。方法建立SPR动物模型,以假手术组和正常对照组进行对照。免疫组化法检测c-fos在模型大鼠腰3-6脊髓内及脑干网状结构抑制区、易化区的表达。结果SPR造成的损伤引起SPR组脊髓后角及脑干网状结构易化区c-fos的显著性表达,与假手术组相比具有显著性差别(P<0.05)。结论c-fos表达情况的检测说明L3-6脊神经后根的传入纤维和脑干网状结构易化区存在着相关功能联系和神经通路联系,SPR术对脊神经后根的切断调整了脑干网状结构易化区的活性,通过相关脑神经核,调整咽喉肌的紧张性使流涎症状缓解。

Objective To find the inside mechanism of SPR remitting the salivation in spastic cerebral palsy.Methods A SPR rat model was made and the false operation group and control group were made at the same time.The sections of the L3~6 spinal cord and facilitation/inhibition area in the brain stem were subjected to c-fos immunohistochemical reaction with ABC method.Results The damage of SPR could make the c-fos expression of the posterior horn in L3~6 and the facilitation section of the brain stem reticular formation in SPR model group was significant stronger than that in the false operation group and control group（P0.05）.Conclusion The expression of c-fos indicate that there is a functional and morphologic relationship between the L3~6 spinal nerve posterior roots and the facilitation section of brain stem reticular formation.SPR cut off the L3~6 spinal nerve posterior roots and this adjusted activity of facilitation section of brain stem reticular formation.