PDMS Microchip Capillary Electrophoresis for Determination of Thiol Compounds 被引量：1

PDMS Microchip Capillary Electrophoresis for Determination of Thiol Compounds

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摘要 A fast and sensitive method for the determination of thiol compounds including homocysteine, glutathione and N-aeetyl-l-cysteine using microchip capillary electrophoresis was proposed. The mierochip was made from an elastomerie material-poly （dimethylsiloxane）（PDMS）. The microehip configureation consists of a top layer of PDMS containing of injection and separation channels, reservoirs and a gold microwire sealed with a bottom layer of PDMS. A gold microwire was used as the working electrode and platinum mierowire was used as counter electrode. The pulsed amperometric detection （PAD） was applied for determination of these thiol compounds. The experiments were carried out with 20 mM MES and 1 mM SDS buffer （pH 6.0）. In order to optimize the conditions, detection and separation potential, pH buffer, injection time, and PAD parameters were studied. Homocysteine, glutathione and N-aeetyl-L-cysteine can be separated and detected in less than 2 rain at the detection potential of ＋0.8 V, separation potential of ＋1200 V, and injection time of 20 s, with good reproducibility and sensitivity. A fast and sensitive method for the determination of thiol compounds including homocysteine, glutathione and N-aeetyl-l-cysteine using microchip capillary electrophoresis was proposed. The mierochip was made from an elastomerie material-poly （dimethylsiloxane）（PDMS）. The microehip configureation consists of a top layer of PDMS containing of injection and separation channels, reservoirs and a gold microwire sealed with a bottom layer of PDMS. A gold microwire was used as the working electrode and platinum mierowire was used as counter electrode. The pulsed amperometric detection （PAD） was applied for determination of these thiol compounds. The experiments were carried out with 20 mM MES and 1 mM SDS buffer （pH 6.0）. In order to optimize the conditions, detection and separation potential, pH buffer, injection time, and PAD parameters were studied. Homocysteine, glutathione and N-aeetyl-L-cysteine can be separated and detected in less than 2 rain at the detection potential of ＋0.8 V, separation potential of ＋1200 V, and injection time of 20 s, with good reproducibility and sensitivity.

作者 Sukanya Viriyatanavirote Weena Siangproh Orawon Chailapakul

机构地区 Program in Petrochemistry and Polymer Science Department of Chemistry Department of Chemistry

出处《Journal of Electronic Science and Technology of China》 2010年第1期78-81,共4页 中国电子科技（英文版）

基金 National Nanotechnology Center (NANOTEC) through grant No. NH-B-22-FN-10-50-06,Asian Development Bank (ADB),Graduate Thesis Grant and Chulalongkorn University for financial support

关键词 Electrochemical detection glutathione homocysteine N-ACETYL-L-CYSTEINE PDMS microehip. Electrochemical detection, glutathione,homocysteine, N-acetyl-l-cysteine, PDMS microehip.

分类号 O658.9 [理学—分析化学]

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