缺氧内皮细胞介导肺动脉平滑肌细胞血小板源性生长因子mRNA表达

Platelet derived growth factor (PDGF) mRNA expression of porcine pulmonary artery smooth muscle cells induced by hypoxic endothelial cells conditioned medium

目的探讨缺氧时肺动脉内皮细胞对肺动脉平滑肌细胞ＰＤＧＦ自分泌的影响。方法实验分３组：无血清培养组、常氧性内皮细胞条件培养液组及缺氧性内皮细胞条件培养液组。应用原位杂交和图像分析技术检测猪肺动脉平滑肌细胞ＰＤＧＦＡ和ＰＤＧＦＢ链ｍＲＮＡ表达。结果无血清培养的肺动脉平滑肌细胞有ＰＤＧＦＡ和ＰＤＧＦＢ链ｍＲＮＡ弱表达；内皮细胞条件培养液明显增加肺动脉平滑肌细胞ＰＤＧＦＡ和ＰＤＧＦＢ链ｍＲＮＡ表达，两者分别为无血清培养组的１．８倍和１．７倍（Ｐ＜０．０１），为常氧内皮细胞条件培养液培养组的１．４倍和１．５倍（Ｐ＜０．０１）。结论缺氧时肺动脉内皮细胞可以介导肺动脉平滑肌细胞ＰＤＧＦ的自分泌。

Objective To study the effect of hypoxic endothelial cell conditioned medium on the autocrine of PDGF in porcine pulmonary artery smooth mucle cells (PASMC). Methods Experimental groups included serum free medium group (control group), normoxic endothelial cell conditioned medium (NECCM) group and hypoxic endothelial cell conditioned medium (HECCM) group. The expression of PDGF A and B chain mRNA was determined by in situ hybridization and image analysis. Results A weak expression for PDGF A and PDGF B mRNA was observed in PASMC cultured in serum free medium (control group); HECCM markedly enhanced the expression of PDGF A and PDGF B mRNA in PASMC, both being about 1.8 fold and 1.73 fold of the control group respectively, and 1.4 fold and 1.5 fold of the NECCM group respectively ( P <0.01).Conclusion PDGF may be an autocrine growth factor of PASMC induced by HECCM and may play an important role in vascular structural remodeling of hypoxic pulmonary hypertension.