连接蛋白43在培养心肌肥大时的表达及其意义

Correlation of expression of connexin 43 and cardiomyocyte hypertrophy

目的研究培养心肌连接蛋白４３（Ｃｘ４３）的表达及与心肌肥大的关系。方法分离培养大鼠心肌细胞，用去甲肾上腺素（ＮＥ）或苯肾上腺素（ＰＥ）诱导心肌肥大，用免疫组化方法观察心肌细胞Ｃｘ４３、增殖细胞核抗原（ＰＣＮＡ）及ｃｄｃ２表达，以图像分析系统检测Ｃｘ４３表达量的变化。结果在培养中加ＮＥ或ＰＥ的心肌细胞，Ｃｘ４３表达明显低于对照组，而其ＰＣＮＡ的阳性率明显高于对照组。ｃｄｃ２阳性率不变。结论在ＮＥ或ＰＥ作用下，培养大鼠心肌细胞中Ｃｘ４３表达下降，与细胞进入Ｓ期有关。

Objective To study the relationship between the expression of connexin 43 and cardiomyocytes hypertrophy. Methods Cardiomyocytes were isolated from newborn Wistar rats. Norepinephrine (NE) or phnylephdrine (PE) were added into the media to induce myocyte hypertrophy. Immunohistochemistry methods were used to identify the expression of connexin 43, proliferating cell nuclear antigen (PCNA) and cycline dependent kinases (cdc 2). The amount of connexin 43 expression was detected by computor imaging analysis system MIPS. Results In the groups which had PE or NE added, the myocytes expressed lower connexin 43 and higher PCNA levels than the control group's. But the expression of cdc 2 was unchanged. Conclusion The reduced expression of connexin 43 was related to its entrance into S phase of the cell cycle. This may explain the mechanism of cardiomyocyte hypertrophy.