外源性透明质酸在愈合中伤口的生化分析

An experiment study in wound healing with exogenous hyaluronic acid

目的探讨外源性透明质酸（ＨＡ）抑制瘢痕形成的作用及其可能机制。方法健康ＳＤ大鼠４５只，复制背部创伤模型，随机分成２％ＨＡ治疗组（Ａ组）、１％ＨＡ治疗组（Ｂ组）、ＰＢＳ对照组（Ｃ组），观察创面大体形态、平均愈合时间和残留面积的大小，测定愈合部位组织蛋白、羟脯氨酸（Ｈｙｐ）、Ⅲ型前胶原（ＰＣⅢ）的含量。结果⑴３组的平均愈合时间为（１５．２±２．０）ｄ、（１４．２±１．６）ｄ和（１３．４±１．４）ｄ，Ａ、Ｂ两组与Ｃ组比较有显著性差异，３组间比较也有显著性差异（Ｐ＜０．０５）。Ａ、Ｂ两组的残留面积与Ｃ组比较有显著性差异，３组间比较也有显著性差异（Ｐ＜０．０５）⑵Ｈｙｐ和ＰＣⅢ含量的测定：Ａ、Ｂ两组Ｈｙｐ含量的均值低于Ｃ组（Ｐ＜０．０１）；而ＰＣⅢ的含量却高于Ｃ组（Ｐ＜０．０１）。结论⑴ＨＡ通过对细胞的调控作用，有效地抑制胶原的合成，提高Ⅲ型胶原含量，使创伤愈合过程中瘢痕形成减少。⑵ＨＡ在创面愈合中抑制瘢痕形成的生物学作用与其浓度成正比关系。

Objective To evaluate the effects and possible mechanisms of hyaluronic acid to inhibit scar formation.Methods 45 health adult SD rats in a dorsal part trauma model were randomly divided into 2% HA treated-group(A group),1% HA treated-group (B group),and PBS treated-group as a control group (C group).The process of healing was observed,the remained wound areas were measured,and a mean wound healing time were calculated.Furthermore,dynamic analysis were performed on local tissure protein,Hyp and PC Ⅲcontents.Results ⑴The mean value of wound healing time was (15.2±2.0)d,(14.2±1.6)d and (13.4±1.4)d respectively.The remained wound areas of control group were much smaller than that in A,B groups ( P <0.05),and there was marked difference among the three groups( P <0.05).⑵The contents of tissue Hyp statistically were lower in HA-treated groups,while the contents of PC Ⅲ were statistically higher comparing to control group ( P <0.01).Conclusion The study indicated that ⑴the exogenous hyaluronan can effectively inhibit collagan synthesis and elvate tissue PC Ⅲ content.As a result,scar formation was decreased.⑵Hyaluronan efficacy presented a dose-dependent manner.