反相高效液相色谱法测定甘油二酯含量研究

Determination of Diacylglycerol Content by Reversed Phase High Performance Liquid Chromatography

用分子蒸馏和柱层析的方法制备了大豆油甘油二酯（DAG）的标准样品,通过高效液相-电喷雾质谱联用（HPLC-ESI-MS）和薄层色谱法（TLC）验证了标准样品的纯度。用反相高效液相色谱（RP-HPLC）在UV=210 nm做大豆油DAG的标准曲线,在质量浓度范围1.0～12.0 mg/mL,大豆油DAG标准曲线线性良好。通过加样回收试验表明,加样回收率在104%～109%之间,RSD在0.69%～5.19%之间。并用该方法分析了大豆油、分子蒸馏DAG样品中的DAG。

Soybean diacylglycerols（DAG） reference material was prepared by combination of molecular distillation and column chromatography.The purity of the reference material was verified by high performance liquid chromatography /electrospray ionization /mass spectrometry and thin layer chromatography.Results：The calibration curve of soybean DAG built by reversed phase-HPLC at UV=210 nm shows a good linear at concentrations in range of 1.0~12.0 mg/mL.Recovery experiments show the recoveries of samples are in range of 104%~109% with RSD in range of 0.69%~5.19%.The contents of DAG in soybean oil and its related DAG samples produced by molecular distillation were analyzed by this method.