阿佛曼链霉菌的^(12)C离子辐照效应及高产菌株选育

Research on Mutation Breeding of High-producing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ^(12)C^(6+)

选用12C6+离子辐照诱变阿维菌素B1a产生菌ZJAV-A1,研究其诱变效应。实验结果表明,12C6+离子辐照剂量50Gy时致死率97%,正突变率最高可达到34.2%。通过12C6+离子诱变处理,结合平板培养基及斜面培养基的正突变菌株筛选,最终获得一株稳定性良好,阿维菌素B1a组分产量稳定在4460—4588μg/ml之间,较出发菌株提高11.1%—14.7%的突变株ZJAV-Y1-203。

Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of ^12C^6＋ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%,when ZJAV-A1 was irradiated by ion beam of 50 Gy ^12C^6＋.After the mutagenesis processing by ion beam of ^12C^6＋ and the screening of orthomutation strains by using plating mediums and slant cultures,the mutant ZJAV-Y1-203 was obtained with the avermectin B1a yield of 4588—4460 μg/ml.Compared with the original strain,the titer was improved 11.1%—14.7%.