补髓健脑方对帕金森小鼠行为学及脑内谷胱甘肽含量与其过氧化物酶活性的影响

Effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats

目的 探讨补髓健脑方对帕金森病小鼠行为学及黑质内还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-PX）的影响。方法选用纯系C57BL老龄小鼠45只，随机分为假手术组、模型组和中药组。采用腹腔注射MPTP法制备帕金森病动物模型。给予中药补髓健脑方灌胃治疗。观察小鼠爬杆实验、悬挂实验评分的变化。榆测小鼠黑质内GSH含量、GSH-PX活性的改变。结果补髓健脑方可提高小鼠的爬杆实验与悬挂实验分数（P〈0．01），并町增加小鼠黑质内GSH含量、增强GSH—PX活性（P〈0．01）。结论补髓健脑方可改善PD小鼠的行为协渊能力，修复PD小鼠黑质神经元的损伤。

Objective To study the effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats. Methods Forty-five the aging pure C57BL rats were divided into a negative injury group, a model group, and a Chinese medicine group. PD animal model were established by injection with MPTP and were treated with Busui Jiannao Prescription by intragastric administration. Score changes of PD model rats in the test of pole-climbing and suspending were observed. GSH content and GSH-PX activity were further measured. Results After treating with Busui Jiannao Prescription, the test scores in pole-climbing and suspending were improved, GSH content and GSH-PX activity in substantia nigra were increased. Conclusion Busui Jiannao Prescription can regulate the behavior coordination and repair the damage of dopaminergic neurons in the substantia nigra of PD model rats.