华蟾素对子宫内膜癌HHUA细胞存活素表达的影响

The effect of cinobufagin on the expression of survivin of HHUA cells in endometrial carcinoma

目的 探讨华蟾素对体外培养的内膜癌HHUA细胞存活素表达的影响。方法体外培养内膜癌HHUA细胞，以不同浓度的华蟾素干预后，应用RT-PCR检测存活素的表达，以流式细胞仪检测细胞凋亡。结果内膜癌HHUA细胞对照绷的存活素mRNA表达为（1．22±0．23），细胞测亡率为（3．11±0．98）％。O25mg／ml华蟾素对内膜癌HHUA细胞存活素mRNA和细胞凋亡率无影响（q=2．89、P〉0．05；q=3．12、P〉0．05）；2．5mg／ml华蟾素干预后，内膜癌HHUA细胞仃活素mRNA表达为（0．73±0．26）、癌细胞凋亡：率为（26．50±6．36）％，与对照组比较有统计学意义（q=5．68、P〈005：q=10．23、P〈0.05）；化25mg／ml华蟾素组与2．5mg／ml华蟾素组比较，存活素mRNA表达、细胞凋亡率均无统计学意义（q=349、P〉0．05；q=4．35、P〉0．05）。结论2．5mg／ml华蟾素可抑制内膜痛HHUA细胞存活素mRNA的表达．抑制癌细胞增殖。

Objective To investigate the effect and significance of cinobufagin on the expression of survivin of HHUA cells in the endometrial carcinoma cultured in vitro. Methods HHUA cells of endometrial carcinoma were cultured in vitro. After being intervened by cinobufagin with different concentration, the expression of survivin was measured by RT-PCR and the cellular apoptosis was tested by flow cytometry. Results In the control group： mRNA expression of survivin of HHUA cells in endometrial carcinoma was （1.22 ±0.23）, cellular apoptotic rate was （2.31 4±0.98）%; cinobufagin of 0.25 mg/ml didn＇t affect the survivin and cellular apoptotic rate （q=2.89, P〉0.05： q=3.12, P〉0.05） ; after being exposed to cinobufagin of 2.5 mg/ml, the expression of survivin was （0.73±0.26）, and cellular apoptotic rate was （26.50±6.36） %, with all of these data being different from the control group significantly （q=5.68, P〈0.05： q=10.23, P〈0.05） ; the actions of cinobufagin of 25 mg/ml on mRNA expression of survivin and apoptotic rate were similar to cinobufagin of 2.5 mg/ml （q=3.49, P〉0.05; q=4.35, P〉0.05） . Conclusion Cinobufagin of 2.5 mg/ml inhibit the mRNA expression of survivin and proliferation of HHUA cells in endometrial carcinoma.