邻苯二甲酸二丁酯孕期暴露仔鼠阴茎组织乳酸脱氢酶B差异表达

Different expression of L-lactate dehydrogenase B chain in the penis of the rat offspring after maternal exposure to DBP

目的:探讨邻苯二甲酸二丁酯(DBP)孕期暴露对仔鼠阴茎组织乳酸脱氢酶B(L-lactate dehydrogenase B,LDH-B)的影响,以进一步探讨LDH-B在DBP导致的尿道下裂中的作用机制。方法:将20只受孕大鼠随机分成2组,实验组10只,对照组10只。在大鼠怀孕第14～18天(GD14～18)2组分别给予灌胃,实验组给予DBP每天800mg/kg染毒孕鼠,对照组给予大豆油每天5ml,妊娠19天(GD19)分别取出仔鼠阴茎组织提取总蛋白,通过Western-blot及免疫组化检测LDH-B的表达。结果:实验组及对照组阴茎组织中均有LDH-B的表达,实验组的相对表达量为0.078±0.02(n=10),对照组的相对表达量为0.137±0.03(n=10),实验组中表达明显减少(P<0.05)。LDH-B主要定位于大鼠阴茎组织细胞的胞浆中。结论:孕期大鼠暴露邻苯二甲酸二丁酯后仔鼠阴茎组织中LDH-B表达明显减少,可能与DBP造成机体缺氧有关。

Objective：To identify and analyze the differential expression of L-lactate dehydrogenase B chain（LDH-B）in the penis of rat offspring after maternal exposure to di-n-butyl phthalate （DBP）. Methods：Twenty pregnant rats were randomly divided into two groups and given DBP by gastric intubation at the dose of 800 mg / kg per day in the experimental group or 5 ml per day soybean oil in the control group from the 14 th to 18 th day of pregnancy. Penises were harvested from the fetal rats of the normal and exposed groups respectively at GD l9 .The expression of LDH-B detected and analyzed by Western blot and immunohistochemistry. Results： LDH-B expressed both in the experiment group and the control group,but the expression level is much lower in the experimental group than in the control group（P 0.05）. LDH-B was mainly located in the cytoplasm of the penis’s tissue. Conclusion：LDH-B protein expressed much lower in the penis of the rat offspring after maternal exposure to DBP than in the penis of the rat offspring without exposure to DBP.