血必净对肺血栓栓塞大鼠P-选择素表达的影响

Effects of Xuebijing injection on the expression of P-selectin of rat with pulmonary thromboembolism

目的:观察大鼠肺血栓栓塞症中血清、肺血管内皮细胞上P-选择素(P-selectin,Ps)的变化,并探讨血必净注射液对其的影响。方法:颈静脉注入自体血凝块制备动物模型。将105只健康雄性SD大鼠按随机数字表法分为对照组、栓塞组和血必净组。每组又分为术后1h、3h、1天、3天、7天5个时间段,每个时间段7只。留取肺组织和血清,观察各组肺组织病理变化,Western blot法检测肺血管内皮细胞上Ps的蛋白表达水平,ELISA法测血清中可溶性P-选择素(sP-selectin,sPs)含量。结果:肺栓塞后病理可见肺动脉中血栓栓塞,炎症反应明显。肺血管内皮上Ps的蛋白表达水平及血清中sPs含量在栓塞后1h开始显著增高(P<0.01)。血必净组经治疗后,病理损伤减轻,肺血管内皮上Ps的蛋白表达水平于1h后下降,与栓塞组比较,有显著性差异(P<0.01),血清sPs含量也下降,与栓塞组比较,1天后差异均有统计学意义(P<0.01或P<0.05)。结论:血必净注射液可能通过降低肺血栓栓塞时Ps表达,从而减轻炎症和凝血纤溶失衡对肺组织的损害,对肺组织具有一定的保护作用。

Objective：To observe and discuss the effects of Xuebijing injection on the expression of P-selectin in serum and on the pulmonary vascular endothelial cells of PTE model rats. Methods：The PTE model was established by intravenous injection of autologous blood clots. One hundred and five healthy male SD rats were randomly divided into control group,PTE model group,and Xuebijingtreated group. Every group were divided into 5 subgroups according to the time of 1 hour,3 hour,1 day,3 day and 7 day after opreation,with 7 rats in each subgroup. Pathological changes of lung tissue were observed,and the expression of P-selectin protein on the pulmonary vascular endothelial cells was evaluated by Western blot. The contents of sP-selectin in serum were detected by ELISA. Results：The notable embolism in pulmonary arteries and inflammatory cell infiltration around alveoli could be found by microscopy. The expression of P-selectin protein on the pulmonary vascular endothelial cell and the contents of P-selectin in serum significantly elevated from 1 hour after embolization （P〈0.01）. Whereas after using Xuebijing injection,the pathological injury was ameliorated.The expression of Ps protein on the pulmonary vascular endothelial cells were significantly decreased from 1 hour after embolization （P〈0.01）. The contents of P-selectin in serum were also decreased and the differences between PTE model group and Xuebijing-treated group were statistically significant from 1 day after embolization （P〈0.01 or P〈0.05）. Conclusion：Xuebijing injection may reduce the damage of inflammation and coagulation-fibrinolysis imbalance to the lung tissue through decreasing the expression of P-selectin in PTE,so it has a certain protective effect to the lung tissue.