HPLC法测定人参、三七颗粒中四种皂苷类成分的含量

Content Determination by HPLC of Four Ginsenosides from Ginseng and Sanchi Granules

目的:建立测定人参、三七颗粒中四种皂苷类成分含量的高效液相色谱方法。方法:Waters Nova-PakC18(150×3.9mm4μm)色谱柱,流动相为乙腈-水溶液梯度洗脱,流速为1.0mL/min,检测波长为203nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.210～1.890μg,0.822～7.398μg,0.203～1.827μg及11.22～10.98μg范围内线性关系良好,相关系数r均大于0.9995。三七皂苷R1的回收率为101.7,RSD=2.3%(n=5);人参皂苷Rg1的回收率为97.4,RSD=1.3%(n=5);人参皂苷Re的回收率为98.9,RSD=1.6%(n=5);人参皂苷Rb1的回收率为100.4RSD=0.9%(n=5)。结论:本方法操作简便,分离效果好,灵敏度高,重复性好,可用于控制人参、三七颗粒的质量。

Objective：To establish a method for the determination of the contents of 4 Ginsenosides in granules of ginseng and sanchi. Methods：The determination was carried out with Waters Nova-Pak C18 column （150 ×3.9mm 4mm）, using acetonitrile -water as the mobile phase （ gradient elution） at a flow rate of 1 ml/min and detected at the wavelength 203 nm. Results ：The calibration curves of No- toginsenoside R1, Ginsenoside Rgl, Ginsenoside Re, Ginsenoside Rbl were in good linearity over the range of 0.210 - 1. 890μg, 0. 822 -7.398μg, 0.203 - 1. 827μg and 11. 22 - 10.98μg, with r =0.9998, 0. 9999, 0. 9996 and 0. 9999, respectively. The average recoveries for Notoginsenoside R1, Ginsenoside Rgl, Ginsenoside Re, Ginsenoside Rbl were 101.7 with RSD （ n = 5） 2.3%, 97.4 with RSD （ n = 5 ） 1.3%, 98.9 with RSD （ n = 5 ） 1.6%, and 100.4 with RSD （ n = 5 ） 0.9%, respectively. Conclusion： The method is simple, sensitive, accurate and reproducible, and can be used to control the qualities of granules of ginseng and sanchi.