三波长光度法测定肉制品中总黄酮含量

Determination of the Total Flavonoids in Meat Product by a Three-wavelength Spectrophotometry

对含有荞麦的新型保健肉制品中总黄酮含量的测定方法进行了系统的研究,建立了一种测定肉制品中总黄酮含量的三波长光度法。结果表明:在选定的测定波长(314、362、409.5nm)下,总黄酮含量在0-50μg/mL内其浓度与吸光度差(ΔA)呈线性关系,其R2=0.999、RSD=1.21%,回收率为98.90%-105.94%,该法用于实际样品分析取得了令人满意的结果。

The determination of the total Flavonoids in the buckwheat meat product was introduced. Three-wave length spectrophotometry was applied. The results indicated： in the designation determination wavelength（314,362, 409.5 nm） , the total flavonoids was 0-50μg/mL, the concentration and absorbent differences were linear. The correlation coefficient R^2 = 0. 999, the RSD was 1.21% , the recovery rate was 98.90% - 105.94%. The result was reliable in the determination of the samples.