超声辅助连续逆流浸取姜黄中姜黄素类物质工艺研究

Study on Ultrasonic Continuous Countercurrent Extraction of Curcumin from Curcuma

通过均匀设计优化多频组合超声辅助连续逆流浸取姜黄中的姜黄素类物质的浸取工艺,探讨了超声频率(25、50、90kHz)、超声强度(0-2400W)、液料比[(15-40)∶1]、温度(30-50℃)、乙醇体积分数(40%-90%)对目标参数的影响。以上述5个因素为影响因素设计了五因素五水平均匀实验,对结果进行偏最小二乘法处理得到:60℃,乙醇体积分数40%,超声频率25kHz,超声强度为600W的单频超声姜黄素浸取浓度最高,达到了0.150mg/mL,同样条件下浸取率达到最大为45.8%,浸取浓度比非超声浸取高33.9%;60℃,乙醇体积分数40%,超声频率(25+50)kHz,超声强度为400W的双频复合超声浸取浓度较高,达到0.144mg/mL,同样条件下浸取率达到最大为45.6%;60℃,乙醇体积分数40%,超声频率25/50kHz,超声强度为200W的双频交变超声浸取浓度为0.145mg/mL,同样条件下改变乙醇体积分数为50%时,浸取率达到最大为42.3%。

Multiple frequency ultrasound wave combinations assisted continuous countercurrent extraction experiment was applied in the study of Curcumin extraction form Curcuma. The experiment was designed through uniform experimental design, and five factors included the frequency of ultrasound （25,50,90 kHz） , the intensity of ultrasound （0 - 2 400 W） , Solid-liquid ratio （ 1/15 - 1/40 ） , temperature （ 30 - 50℃ ） and the ethanol concentration （40% - 90% ）. The results showed that the highest extracting concentration was 0. 150 mg/mL with the highest extracting ratio of 45.8% under the conditions of Single-frequency ultra-sound wave assisted at 60℃, 40% alcohol, 25 kHz and 600W. This concentration was 33.9% higher than that of no ultrasound wave assisted condition. In the condition of 60℃ ,25 ＋ 50 kHz, 40% of alcohol and the intensity 400W, the extracting concentration was 0. 144 mg/mL, the extracting yield was 45.6%. In the condition of double-frequency alternating ultrasound wave assisted, the concentration reached to the peak of 0. 145 mg/mL,under 60℃ , 25/50 kHz , 200 W , 40% alcohol.