不同年龄段健康儿童血清肌酸激酶同工酶(CK-MB)临床评估

Clinical evaluation of serum creatine phosphokinase isoenzyme in healthy children at different ages

目的比较不同年龄段健康儿童血清肌酸激酶同工酶(CK-MB)活性和质量差异,并对其临床合理应用进行探讨。方法选取184例健康儿童,按年龄阶段进行分组,同时选取30例健康成人设为对照组,分别采用酶免疫抑制法测定血清CK-MB活性,双抗体夹心法测定血清CK-MB质量。结果各健康儿童组和成人组CK-MB活性和质量呈明显正相关;儿童组CK-MB活性和质量随年龄增长呈下降趋势,趋近成人水平;各组质量检测均在正常参考范围,而低年龄儿童组CK-MB活性略高于临床正常值。结论临床医生在参考CK-MB活性和质量检测结果进行病情判断时,要考虑年龄因素;在临床诊断中CK-MB质量测定更优于活性测定。

Objective To compare the different activity and quality of serum creatine phosphokinase isoenzyme （CK-MB） in healthy children at different ages and to study its rational clinical application. Methods A total of 184 healthy children were divided into different age groups and 30 healthy adults served as a control group. Activity and quality of their serum CK-MB were detected by inhibition enzyme immunoassay and double antibody sandwich enzyme-link immunoassay, respectively. Results A positive correlation was observed between the activity and quality of serum CK-MB in the different groups. In children group, the activity and quality CK-MB decreased with the increasing age, almost reaching those in the adults. The CK-MB quality was normal in the different age groups. However, the CK-MB activity was slightly higher than normal in children at a younger age. Conclusion It is necessary to consider the age when the Clinician determines the condition of patients based on the CK-MB activity and quality. Moreover, CK-MB quality assessment is better than the activity assessment in clinical diagnosis of patients.