转染SV40永生化基因正常成人肝细胞的形态观察

Morphology of SV40 transfection gene-immortalized liver cells in normal adults

目的了解转染SV40基因对正常成人肝细胞形态结构及特殊染色的影响。方法采用本组构建的含SV40永生化基因的正常成人肝细胞(rSSR#69/HL-7702肝细胞),以HE染色法、糖原染色法(PAS)和葡萄糖-6-磷酸酶染色(G-6-Pase)法,在光镜下观察该细胞的形态特征,以电镜法观察该细胞的超微结构。结果:光镜下比较转染前、后的正常成人肝细胞形态无明显差异;PAS染色均可见胞浆内有粉红色的糖原颗粒,G-6-Pase染色均可见胞质内有铁黑色颗粒;电镜下比较转染前、后的正常肝细胞的超微结构亦无统计学差异。结论:转染SV40永生化基因的正常成人肝细胞的形态结构与转染前无明显差异,仍具有糖原合成及葡萄糖代谢的能力。

Objective To study the effect of SV40 gene transfection on morphological structure of liver cells in normal adults and its special staining. Methods Liver cells （rSSR # 69/HL-7702 liver cells） containing SV40 gene from normal adults were stained with HE, glycogen and glucose-6-phosphatase （G-6-Pase）, and their morphological features and ultra-structure were observed under a light microscope and an electron microscope, respectively. Results No significant difference was found under light microscope in morphology of liver cells from normal adults before and after transfection. Glycogen staining showed glycogen pink granules in cytoplasm and G-6-Pase staining showed black iron particles in cytoplasm. Also, no significant difference was observed in ultrastructure of liver cells under electron microscope before and after transfection. Conclusion The morphological structure of liver cells from normal adults is not significantly different before and after SV40 transfection gene-immobilized liver cells. SV40 transfection gene-immobilized liver cells are able to synthesize glycogen and metabolize glucose.