卡介菌多糖核酸对支气管哮喘小鼠气道反应性及气道炎症的影响

Effects of bacillus calmette-guerin polysaccharide nucleotide on airway inflammation, airway hyperresponsiveness in asthmatic mouse model

目的探讨卡介菌多糖核酸对哮喘小鼠气道反应性及气道炎症的影响。方法选择Balb／c小鼠，以卵白蛋白致敏激发建立小鼠哮喘模型，设立卡介菌多糖核酸组、正常组、哮喘组。卡介菌多糖核酸按剂量具体分为1μg，10μg，100μg亚组，均在第一次抗原致敏前7d腹腔注射给药。末次激发后48h采用美国Buxco公司小鼠整体体积扫描记法测定气道反应性，以乙酰甲胆碱各浓度激发时增强的呼吸问歇（EnhancedPause，Penh）表示。以PC100[气道反应性升高为生理盐水（NS）值2倍时的Mch激发浓度]及Penh／NS％max综合评价气道反应性。收集支气管肺泡灌洗液，涂片后苏木素-伊红染色计数嗜酸粒细胞比例，肺组织病理检测。结果1pg组PCI00（13．2±6．9）g／L、10μg组（11．8±5．58）g／L与哮喘组（5．97±1．73）g／L相比，P〈0．01表示差异有统计学意义；1μg、10μg组Penh／NS％max分别为（623．22±252．39）％、（519．71±200．41）％，显著低于哮喘组（1306．83±540．46）％，P〈0．01。10／zg组BALF嗜酸粒细胞百分比为（42．75±7．44）％显著低于哮喘组（57．25±13．2）％，P〈0．01，1μg组、100μg组BALF嗜酸粒细胞百分比与哮喘组相比，差异无统计学意义。结论卡介菌多糖核酸可以显著抑制哮喘小鼠的气道高反应性及气道炎症。

Objective To investigate the effect of Bacillus Calmette-Gu6rin polysaccharide nucleotide （BCG-PSN） on airway hyperresponsiveness and airway inflammation in asthmatic mouse model. Methods Balb/c mice at 6 weeks of age were sensitized with ovalhumin （OVA） by intraperitoneal injection on day 0,7 and 14,then challenged with OVA by intranasal administration on day 28,29,30 for asthmatic model. The mice of control group didn＇t receive any treatment. A pretreatment of 1 μg, 10 /Lg, 100 μg of BCG-PSN by intraperitoneal injection were done at 7 days before the first sensitization. Bronchial hyperresponsiveness,bronchoalveolar lavage fluid and histological changes in the airways were examined 48 hours after the final challenge. The data was processed statistically with one-way analysis of variance in SPSS package （version 13.0）. Results Airway hyperresponsiveness of mice administrated with BCG-PSN at the dose of 1 μg,10μg were significantly lower than the asthma group （ P 〈0.05）. The treatment with BCG-PSN at the dose of 100 μg didn＇t decrease airway hyperresponsiveness significantly. Airway eosinophilia of 10 μg group was significantly lower than the asthma group [（42. 75±7.44）% vs （57.25±13.19）%, P 〉0.01]. Airway eosinophilia of 1 μg group,100 μg group [（49.13±4.99）% vs （52.75 ± 7.34）%] both have no significant difference compared with asthma group （ P〉 0.05）.Conclusions BCG-PSN can inhibit the airway inflammation and decrease the hyperresponsiveness in asthmatic mouse model.