高效液相色谱法测定荜茇中胡椒碱的含量

HPLC Determination of Piperine in Fructus Piperis Longi

目的：建立HPLC法测定荜茇中胡椒碱含量的方法。方法：采用Diamonsil C18色谱柱，以甲醇－水（77：23）为流动相，流速1．0mL·min^-1，检测波长343nm。结果：胡椒碱线性范围为0．04116－0．4116μg（r=1．0000），平均加样回收率为103．2％，RSD为1．3％。结论：本方法操作简便、快速、准确、灵敏度高，重复性好。

Objective ： To establish HPLC method for determine of Piperine in Fructus piperis longi. Methods ： Using ODS column, Methanol -Water （77： 23）as the mobile phase at flow rate of 1. OmL ·min^-1, wavelength of the detection was 343 nm. Results ： Liner range of Piperine was 0.04116 μg -0. 4116 μg （ r = 1. 0000） ; the recovery was 103.2% （ RSD = 1.3% ）. Conclusion ： This method is simple, rapid, accurate, highly sensitive and well reproducible.