摘要
目的:探讨蝙蝠葛酚性碱(PAMD)抗胰腺癌作用靶点。方法:右腋皮下接种胰腺癌(BXPC-3)肿瘤细胞,经腹腔注射PAMD,连续用药21天,采用荧光定量PCR(Real-time PCR)技术检测肿瘤k-ras mRNA表达的情况。结果:PAMD高、中、低剂量均能下调BXPC-3荷瘤小鼠瘤组织中K-ras mRNA表达量,与模型对照组比较,差异具有统计学意义(P<0.01)。结论:说明PAMD对BXPC-3荷瘤小鼠具有明显的抑瘤作用。
Objective：To research PAMD action target against pancreatic cancer. Method：Nude mice were inoculated with pancreatic cancer BXPC -3 cells under right armpit and injected with PAMD by abdominal cavity about 21days, and k -ras mRNA expression was detected by Real -time fluorescent quantitative PCR. Results：The high, moderate, and low dose groups of PAMD can all reduce k - ras mRNA expression of nude mice inoculated with pancreatic cancer BXPC -3 cells more obviously than model control group（P 〈 0. 01 ）. Conclusion：which shows the obvious anti -tumor effect of PAMD.
出处
《中医药信息》
2010年第2期89-92,共4页
Information on Traditional Chinese Medicine
基金
2007黑龙江省自然科学基金课题(D200675)
