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p21^(WAF1/CIP1/SDI1)在大鼠肾脏组织的增龄性变化及意义

Expression of p21^(WAF1/CIP1/SDI1) in aging rat kidneys
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摘要 目的研究p21野生型p53活化片段1/细胞周期蛋白依赖性激酶影响蛋白1/衰老细胞衍生抑制剂1(p21WAF1/CIP1/SDI1)在大鼠肾脏中随年龄增长的表达变化规律。方法取3月龄、12月龄及24月龄健康雄性Wistar大鼠肾组织进行衰老相关β-半乳糖苷酶(SA-β-gal)活性染色,TUNEL法检测细胞凋亡,采用逆转录多聚酶链反应(RT-PCR)和Western印迹法(Westernblotassay)分别在基因及蛋白质表达水平上检测肾脏组织中p21WAF1/CIP1/SDI1的表达变化,并用免疫组化法检测p21WAF1/CIP1/SDI1在肾脏组织中的表达与定位。结果大鼠肾脏组织SA-β-gal活性随年龄增长逐渐增强,凋亡细胞也随年龄增长逐渐增加(P<0.05);p21WAF1/CIP1/SDI1mRNA表达随年龄增长逐渐增强,不同月龄比较差异有统计学意义(P<0.05)。Western印迹亦显示p21WAF1/CIP1/SDI1蛋白表达随鼠龄增加逐渐增强(P<0.05)。免疫组化结果显示,p21WAF1/CIP1/SDI1蛋白表达于大鼠肾小球足细胞,其在肾小管与间质细胞中也有表达,且随年龄增长表达增加(P<0.05)。结论p21WAF1/CIP1/SDI1在大鼠肾脏组织中的表达随年龄增加而增强,可作为肾脏组织中重要的衰老指标。 ObjectiveTo investigate the effect of p21WAF1/CIP1/SDI1 on the renal lesions in aging kidneys in rats. MethodsMale Wistar rats at the ages of 3, 12, 24 months were used in this study. The renal tissues were processed for senescence associated βgalactosidase （SA-β-al） staining. TUNEL assay was used to detect apoptosis of renal tubular epithelial cells. The expression and location of p21WAF1/CIP1/SDI1 in kidneys were studied by immunohistochemistry. The mRNA and protein levels of p21WAF1/CIP1/SDI1 were detected by Western blot and RTPCR, respectively. ResultsThe expression of neutral β-galactosidase activity was increased in aging kidneys. The number of apoptotic cells increased in kidney with advancing age（P〈0.05）. The p21WAF1/CIP1/SDI1 staining appeared in the podocyte and interstitium in kidney. The staining intensity of p21WAF1/CIP1/SDI1 in kidneys in old animals was markedly increased, as compared to that in young and middleaged rats （P〈0.05）.The mRNA level of p21WAF1/CIP1/SDI1 was significantly increased in aging kidneys, as compared to that at the age of 3 and 12 months, meanwhile the p21WAF1/CIP1/SDI1 proteins expression was dramatically increased with advancing age （P〈0.05）. Conclusionsp21WAF1/CIP1/SDI1 expression increases with the aging of kidneys, which may serve as an important marker of organismal aging.
作者 卓莉 刘伏友 蔡广研 汪杨 师锁柱
机构地区 中国人民解放军总医院肾内科全军肾脏病研究所暨重点实验室 中南大学湘雅二医院肾病内科 中南大学肾脏病研究所
出处 《实用老年医学》 CAS 2010年第2期113-117,共5页 Practical Geriatrics
基金 国家重点基础研究发展计划"973"资助项目(2007CB507400) 国家自然科学基金资助项目(30771014) 中国博士后科学基金(20090450200)
关键词 p21WAF1/CIP1/SDI1 肾脏 衰老 大鼠 p21WAF1/CIP1/SDI1 kidney aging rat
分类号 Q75 [生物学—分子生物学]
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参考文献15

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实用老年医学
2010年 第2期

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