3种林木病原腐朽菌生长特性研究

Growth Characteristics of Three Pathogenic Wood-decaying Fungi

室内培养条件下,研究了温度、光照、碳源、氮源、NaCl、pH值对木层孔菌Ps菌株、裂褶菌Sc菌株和香栓孔菌Ts菌株生长速度的影响。结果表明：3种病原菌生长的适宜温度为20~35℃,Ts属于中温菌,Ps和Sc属于高温菌;Ps在全黑暗条件下生长较快,但Sc和Ts在不同光照条件下的生长差异不显著;Ps和Sc以果糖为最佳碳源,而Ts在麦芽糖培养基上生长最快;病原菌在以有机氮为氮源的培养基上生长较好,在无碳、无氮培养基上也能微弱生长,但在尿素培养基上都不生长;病原菌在pH4.7~6.9的偏酸性条件下生长较好;NaCl胁迫能抑制菌丝生长,3种病原菌最大耐受盐度分别为Ps 4%、Sc 9%、Ts 3%。

Effects of temperature,light,carbon source,nitrogen source,NaCl,pH value were studied on the colony growth of three pathogens： Phellinus sp.（Ps） strain,Schizophyllum commune（Sc） strain and Trametes suaveolens（Ts） strain.The results showed that the pathogen could grow well from 20~35℃,Ts belongs to moderate temperature fungi,while Ps and Sc belong to high temperature fungi;though Ps grew faster in darkness,light and darkness had no significant influence on Sc and Ts;fructose was the optimum carbon source for Ps and Sc,while maltose was the best for Ts;they grew well within organic nitrogen but could hardly grow within urea,however,they could grow up in conditions of no carbon source or no nitrogen source;pathogens prefer weak acid condition and the suitable pH value ranging from 4.7 to 6.9;NaCl stress could restrain mycelium growth,the most tolerance salt concentration was Ps 4%,Sc 9%,Ts 3%.