矮生紫薇的组织培养与再生技术研究

Research on Tissue Culture and Regeneration Technology of Short Crape Myrtle(Lagerstroemia indica cv. Petite Pinkie)

[目的]筛选矮生紫薇(Lagerstroemia indica cv.Petite Pinkie)组织培养的最佳培养基,建立矮生紫薇植株再生体系。[方法]将灭过菌的矮生紫薇种子分别接种于7种无菌苗新诱导培养基上,将获得的无菌苗新生芽接种于8种快繁培养基上,当无根苗长至2～4cm时切去基部组织,接种于8种生根培养基中培养。[结果]不同的含有一定浓度激素的培养基中的矮生紫薇种子的发芽率明显高于不含任何激素的培养基。矮生紫薇试管苗适宜的诱导培养基为MS+6-BA1.00mg/L+NAA0.10mg/L;适宜的快繁培养基为MS+6-BA2.00mg/L+KT1.00mg/L+NAA0.01～0.05mg/L;适宜的生根培养基为MS+NAA0.50mg/L。[结论]该研究为木本矮生紫薇的组织培养及遗传转化提供了技术支持。

[Objective] The research aimed to screen out the optimum media for the tissue culture of Lagerstroemia indica cv.Petite Pinkie and set up its plantregeneration system.[Method] The sterilized Lagerstroemia indica cv.Petite Pinkie seeds were inoculated respectively on 7 kinds of nduction media for inducingasepsis seedling.The inducingasepsis seedling was inoculated respectively on 8 kinds of fast propagating media.When they were 2-4 cm,the rootless seedlings were inoculated respectively on 8 kinds of rooting media.[Result] The germination rate of Lagerstroemia indica cv.Petite Pinkie seeds on media adding with different concentration hormones was obviously higher than that in media without the hormone.The suitable medium for inducing the Lagerstroemia indica cv.Petite Pinkie seedlings were MS＋6-BA 1.00 mg/L＋NAA 0.10 mg/L.The optimum medium for prolife ration was MS＋6-BA 2.00 mg/L＋KT 1.00 mg/L＋NAA 0.01-0.05 mg/L.The optimum medium for rooting was MS＋NAA 0.50 mg/L.[Conclusion] The study can provide technological support for the tissue culture and genetic transformation of Lagerstroemia indica cv.Petite Pinkie.