杜仲叶环烯醚萜类化合物提取工艺研究

Study on the Extraction of Iridoids from Eucommia ulmoids Leaves

[目的]研究杜仲叶中总环烯醚萜类化合物的提取工艺,考察提取条件对有效物质含量的影响。[方法]用正交法优选最佳提取条件,用比色法测定其含量。[结果]杜仲叶中总环烯醚萜类化合物的最佳提取条件为:用1:12同液质量比的85%乙醇作为提取溶剂,于80℃热回流提取3次,每次45min,提取率为0.507%。桃叶珊瑚苷在0.1016～0.8128mg范围内呈良好线性关系,线性方程为:Y=1.0731X-0.0474,相关系数r=0.9970,平均加样回收率为103.18%,RSD为1.94%。[结论]该法操作简便、快捷,适用于杜仲叶中环烯醚萜类化合物的含量测定。

[Objective] The aim was to study the extraction conditions of iridoids from Eucommia ulmoids leaves and investigate effects of extraction condition on effective substance contents.[Method] Orthogonal test was used to optimize the best extraction conditions,and the content of iridoids was determined by colorimetry method.[Result] The optimum extracting factors were 12 times of 85% alcohol,refluxing and extracting for 45 min,for 3 times at 80 ℃.The extraction rate of iridoids was 0.507%.The linear relationship of aucubin was at a range of 0.101 6-0.812 8 mg,the linear equation was：Y=1.073 1X-0.047 4,r=0.997 0.The average recovery was 103.18% with RSD 1.94%.[Conclusion] The method is simple and convenient,which could be used for content determination of iridoids in E.ulmoids leaves.