膏桐叶片再生植株体系的建立

Research on the Establishment of the System of the Plantlet Regeneration from Jatropha curcas Leaf Culture

[目的]研究不同激素组合对离体叶片再生体系建立的影响。[方法]选用6-BA与KT为分裂素,以无菌苗的离体叶片为外植体,在MS培养基中进行不定芽诱导,经增殖培养后,用生长素NAA、IBA促进生根,从而判断不同激素对不同外植体诱芽、增殖、生根的影响。[结果]在MS培养基中,单独使用分裂素6-BA或者KT与生长素NAA的组合都不能较好地对叶片进行不定芽的诱导。在同时添加1.00mg/L的6-BA和KT以及0.03mg/L的NAA后对不定芽的诱导效果最好,诱导率达90%;加入0.50mg/L的6-BA、0.50mg/L的KT和0.02mg/L的NAA后,不定芽的增殖和伸长效果较好;在加入0.10mg/LNAA的MS培养基上生根效果最佳。[结论]以MS培养基用适量浓度植物激素诱导膏桐离体叶片,可建立稳定的再生体系,为膏桐的组培扩繁体系提供了一种新的选择。

[Objective] The effect of the different combinations of hormones on plantlet regeneration from Jatropha curcas leaf culture in vitro was studied.[Method] The adventitious bud was induced from the leaf as explants cultured in vitro in MS medium containing 6-BA and KT as cytokinin and the adventitious buds after proliferation culture were rooted in the medium with NAA and IBA as cellular auxin.The effect of the different hormones or their combination on the induction of adventitious bud,proliferation and rooting in leaf culture as explants was determined.[Results] The results showed that the adventitious bud was not introduced from Jatropha curcas leaf culture in the MS medium containing 6-BA or the combination of KT and NAA and the best introduction efficiency of the adventitious bud from leaf culture was in the medium added with 6-BA of 1.00 mg/L,KT of 1.00 mg/L and NAA of 0.03 mg/L,which induction rate of the adventitious buds was 90%;the proliferation and elongation of the adventitious bud were with better-performing in the medium added with 6-BA of 0.50 mg/L,KT of 0.50 mg/L and NAA 0.02 mg/L;and the best rooting results was from the MS medium added with NAA of 0.10 mg/L.[Conclusion] The stable system of plantlet regeneration from Jatropha curcas leaf culture in vitro could be established in the application of MS medium with the appropriate concentration of plant growth regulator,which provided a new choice for the multiplication of Jatropha curcas by means of the technique of tissue culture.