星形细胞肿瘤中MDM2蛋白与突变型p53蛋白的表达及其相关性研究

Expression of MDM2 protein and mutant p53 protein and their relationship in astrocytic neoplasms

目的：探讨ｍｄｍ－２基因表达和ｐ５３基因突变在星形细胞肿瘤发生发展中的作用及两者的相关性。方法：采用ＳＡＢＣ免疫组化法对１２０例星形细胞肿瘤进行检测。结果：在分化不良性星形细胞瘤及多形性胶质母细胞瘤中，ＭＤＭ２蛋白的阳性率分别为３０．００％和３５．２９％，突变型ｐ５３蛋白的阳性率分别为３５．００％和４４．１２％，均明显高于高分化星形细胞瘤（４．３５％和６．５２％）；突变型ｐ５３蛋白染色阴性的星形细胞肿瘤中，ＭＤＭ２蛋白阳性率（２６．１５％）明显高于其染色阳性的肿瘤（９．３８％）。结论：ＭＤＭ２蛋白及突变型ｐ５３蛋白在星形细胞肿瘤中的表达均与该肿瘤的分化程度有关；ｍｄｍ－２基因扩增及产物ＭＤＭ２蛋白过度表达。

Purpose To demonstrate the role of MDM2 protein and mutant p53 protein in astrocytic neoplasms formation and progression and their relationship. Methods By using the SABC immunohistochemical technique, 120 cases of astrocytic neoplasms were detected. Results The positive rates of the two proteins were increased significantly in anaplastic astrocytoma and multiform glioblastoma as compared with those in low grade astrocytoma. Conclusion The expression of the MDM2 protein and mutant p53 protein is significantly related to the grade of differentiation of the tumor. The amplification and overexpression of mdm 2 gene may be an alternative molecular mechanism in the formation of astrocytic neoplasms with mutant p53 protein negative stained.