碱性成纤维细胞生长因子、纤维连接蛋白在肺炎支原体感染中的临床意义

The clinical value of determination of basic fibroblast growth factor and fibronectin in patients infected with mycoplasma pneumoniae

目的探讨碱性成纤维细胞生长因子(bFGF)、纤维连接蛋白(FN)在肺炎支原体感染患者中的临床意义。方法对我院2007年1月～2009年6月108例慢性咳嗽患者行支气管肺泡灌洗液(BALF)肺炎支原体-聚合酶链反应(PCR)检测。根据检测结果,分为实验组72例(肺炎支原体阳性)与对照组36例(肺炎支原体阴性)。对两组患者进行支气管镜肺活检,通过酶联免疫吸附试验(ELISA)方法检测bFGF水平,Western blot方法检测FN水平。结果肺炎支原体感染组bFGF及FN水平明显高于对照组,差异有统计学意义(P<0.05)。结论肺炎支原体肺部感染可使肺组织bFGF水平增加,而bFGF可进一步诱导FN表达增加。bFGF、FN检测对肺炎支原体所致肺间质纤维化患者早期诊断、病情动态变化、治疗有一定的参考价值。

Objective To explore the clinical value of determination of basic fibroblast growth factor（bFGF） and fibronectin（FN） in patients infected with mycoplasma pneumoniae. Methods 108 patients of chronic cough were detected mycoplasma pneumoniae used by polymerase chain reaction （PCR） in bronchial lavage fluid （BALF）. All patients were divided into an experimental group （ mycoplasma pneumoniae-positive） and a control group （Mycoplasma pneumoniae negative）. Basic fibroblast growth factor（bFGF） and fibronectin （FN） were detected with ELISA and western bolt. Results The concentration level of bFGF and FN in the experimental group was significantly higher than that in the control group （ P 〈 0. 05 ） （ bFGF ： 110.6 ±11.9 vs 34.4 ± 4. 0 pg/ml, P = 0. 0003 ）. Conclusion Pulmonary infection with mycoplasma pneumoniae can lead to up-regnlation of bFGF expression, which promotes secretion of FN. bFGF and FN might play an important role in the pathogenesis of pulmonary interstitial fibrosis after mycoplasma pneumoniae. They could be applied to reflect the patient＇s condition and to assess prognosis.