小肠脂酸结合蛋白基因与2型糖尿病患者体脂含量及分布的相关性

The relation of intestinal fatty acid binding protien gene Ala54Thr variation to general and regional adipose depots in type 2 diabetes mellitus

目的观察小肠脂酸结合蛋白基因（ＦＡＢＰ２）密码子５４变异与体脂含量及分布的关系。方法１６５例中国人，糖耐量正常（ＮＧＴ）者８６人，２型糖尿病患者（ＤＭ）７９人。用ＰＣＲ／ＨｈａＩ酶解检测ＦＡＢＰ２密码子５４变异的等位基因———苏氨酸５４（Ｔｈｒ５４）及丙氨酸５４（Ａｌａ５４）。比较ＮＧＴ及ＤＭ组间及组内体脂含量亚组（ＢＭＩ）及体脂分布亚组（核磁共振局部体脂测定）间的ＦＡＢＰ２密码子５４频率分布。结果ＤＭ股部皮下脂肪（ＦＡ）亚组间ＦＡＢＰ２密码子５４变异基因型频率分布有显著差异（χ２＝１１．４６，Ｐ＝０．００３）。表现为ＦＡ＜７５ｃｍ２者Ｔｈｒ５４（＋）基因型频率显著增高（χ２＝１０．１１，Ｐ＝０．００１；比数比４．６２），及Ｔｈｒ５４等位基因型频率显著增高（χ２＝５．３７９，Ｐ＝０．０２０）。ＦＡ相关因素的多元逐步回归分析中见到除性别（Ｐ＝０．０００１）、ＢＭＩ（Ｐ＝０．０００１）及血总胆固醇（Ｐ＝０．００４）外，ＦＡＢＰ２亦是独立相关因素（Ｐ＝０．００３）。ＦＡＢＰ２相关因素的Ｌｏｇｉｓｔｉｃ回归分析中，仅见ＦＡ与之相关（Ｐ＝０．０２９）。结论ＦＡＢＰ２与体脂分布相关。因为ＤＭ不仅有腹内脂肪增多，且有股部?

Objective To investigate the association between the codon 54 variation of intestinal fatty acid binding protein (FABP2) gene and the amount and the distribution of adipose depots. Methods In 165 Chinese subjects 86 with normal glucose tolerance (NGT), and 79 with type 2 diabetes (DM) . The alleles of the codon 54 variation of FABP2 gene allele threonine 54 (Thr54) and allele alanine54 (Ala54) were detected by using PCR/Hha I digestion. The frequencies of the FABP2 codon 54 variation were compared between NGT and DM groups, and also between body fat subgroups (expressed as BMI) and body fat distribution subgroups (expressed as regional adipose tissue area measured by magnetic resonance imaging) within NGT and DM groups. Results The genotype frequencies of FABP2 codon 54 variation were significantly differentbetweenfemoralsubcutaneousadiposetissue(FA)subgroupsinDMsubjects (χ 2=11.46,P=0.003). DM subjects with FA<75 cm 2 had higher frequency of Thr54 (+) genotype (χ 2=10.11,P=0.001; odd ratio=4.62) and higher frequency of Thr54 allele (χ 2=5.379,P=0.020; odd ratio=2.36). Stepwise regression analysis showed that in addition to sex (P=0.0001), BMI(P=0.0001) and serum total cholesterol (P=0.004), FABP2 codonvariationwasalsoanindependentfactoraccount for FA (P=0.003). Logistic regression analysis revealed that only FA was associated with FABP2 codon 54 variation (P=0.029). Conclusion FABP2 gene was correlated with body tissue adipose distribution. In DM, not only increased intra abdominal fat, but also decreased femoral fat were observed. This study showed that the decreased femoral fat depot in DM subjects was related to FABP2 Thr54 (+) genotype, which suggests that FAPB2 gene may take part in the pathophysiological process of diabetes mellitus.