摘要
试验目的在于选出合适的‘火鸟’蝴蝶兰花梗组织培养的培养基配方,从而建立其组织培养技术体系。试验采用带腋芽的花梗段来诱导丛生芽,继而进行继代增殖培养,最后诱导生根。对培养的各阶段培养基及激素配比进行研究,结果表明,较好诱导培养基为MS+6-BA5.0mg/L+NAA0.05mg/L,继代增殖培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,生根培养基为1/2MS+NAA0.3mg/L。
The purpose of this study is to choose the best media prescription, and set up the culture system. The experiment used the footstalk to induce the shoot cluster, mutiplication culture, and induce to root. The results showed, the better inducement media is MS+6-BA5.0mg/L+NAA0.05mg/L, mutiplication media is MS+6-BA2.0mg/L+NAA0.2mg/L, rooting media is 1/2MS+NAA0.3mg/L.
出处
《中国园艺文摘》
2010年第4期7-9,共3页
Chinese Horticulture Abstracts
关键词
蝴蝶兰
花梗腋芽
组织培养
Phalaenopsis amabilis
footstalk
culture