不同浓度IBA对‘中梨一号’生根的影响

A Study on the Effects of Different IBA Concentration on Rooting of 'Zhong Li Yi Hao'

以‘中梨一号’试管苗为试材,探讨不同浓度的IBA(0.5、1、1.5、2、3、4、5、6、7mg/L)对其生根的影响。试验结果表明:诱导试管苗生根的最佳培养基为1/2MS+IBA(1mg/L)+蔗糖(25g/L)+琼脂(6g/L),生根率达47.62%,且生根苗发育良好,根系生长健壮。生根苗长至5片叶时,移栽到保水透气好的蛭石中,其移植成活率80%以上。

Tissue culture which was used in ＇Zhong Li Yi Hao＇, was to explore the effect of different IBA concentration （0.5, 1.5, 2, 3, 4, 5, 6, 7 mg/L） on rooting. And the result showed： the optimum culture medium that induced rooting of plantlet was 1/2MS ＋ IBA （1mg/L） ＋ suerose（25g/L）＋ agar（6g/L）, the rooting rate was up to 47.62%, and the seedling developed well and had strong root. When plantlet had five leaves, it was transplanted into vermiculite that had fine water retentation and permeability, and the transplanting survival rate was above 80%.