摘要
多性状的分子标记辅助选择具有重要的育种应用价值。研究先应用与水稻抗稻瘟病基因Pi25紧密连锁的分子标记对394个重组自交系进行筛选,再应用育性恢复基因连锁标记检测中选株系,获得20份候选恢复系和27份候选保持系。经田间接种稻瘟病菌、考查测交F1育性,表明利用该研究策略可以同步对多个性状进行选择,提高杂交稻亲本选育的效率。
Marker-assisted selection for muhiple traits is of great significance in rice breeding. A total of 394 rice recombinant inbred lines were screened with DNA markers tightly linked to rice blast resistance gene Pi25. Lines carrying the resistant homozygote were genotyped with DNA markers tighty linked to genes for fertility restoration. Twenty candidate restorer lines and 27 candidate maintainer lines were selected. Blast resistance and fertility restoration of the 47 lines were tested with phenotypic evaluation. Results suggested that the step-by-step approach used in this study is feasible for marker-assisted selection of multiple traits.
出处
《福建稻麦科技》
2010年第1期1-3,共3页
Fujian Science and Technology of Rice and Wheat
基金
国家863计划课题"强优势水稻杂交种的创新与应用"(2009AA101101)
国家超级稻新品种选育与示范项目课题"超级稻分子育种研究"(2009-6)
关键词
杂交稻亲本
分子标记辅助选择
稻瘟病
育性恢复
parental lines of hybrid rice
marker-assisted selection
blast resistance
fertility restoration
