摘要
目的:研究cGMP依赖性蛋白激酶Ⅱ(cGMP dependent protein kinaseⅡ,PKGⅡ)对胃癌细胞株BGC-823迁移活动的影响,并对其作用机制进行初步探讨。方法:以携带PKGⅡ基因的腺病毒结构Ad-PKGⅡ感染胃癌BGC-823细胞,用蛋白质印迹法及免疫荧光检测感染病毒后PKGⅡ的表达。用特异性PKGⅡ激活剂8-pCPT-cGMP作用感染病毒的细胞,通过Boyden槽迁移率、刮除迁移分析法分析PKGⅡ高表达和活性增高对Ras同源性蛋白A(Ras homologA,RhoA)激动剂溶血磷脂酸(lysophosphatidic acid,LPA)诱导的胃癌细胞迁移的影响。结果:胃癌细胞株BGC-823在感染Ad-PKGⅡ后高表达PKGⅡ,经cGMP类似物8-pCPT-cGMP激活后,使RhoA活化诱导的细胞迁移活动受到明显抑制,与对照组比较差异有统计学意义(P<0.05)。结论:PKGⅡ能明显抑制胃癌细胞株BGC-823的迁移。
Objective:To investigate the effect of cGMP dependent protein kinase Ⅱ on the gastric cancer cell migration.Methods:An adenovirus encoding PKG Ⅱ gene was used to infect BGC-823 cells.PKG Ⅱ expressions were detected with Western blotting and immunofluorescence microscopy.The cells were treated with cGMP analogues 8-pCPT-cGMP to activate PKG Ⅱ and the effect on Ras homolog A (RhoA) agonist lysophosphatidic acid (LPA) induced cell migration was examined by Boyden scrape-migration assay and chamber migration assay.Results:The PKG Ⅱ expression in gastric cancer cell BGC-823 was increased after Ad-PKG Ⅱ infection.When the cells were treated with 8-pCPT-cGMP,there was a significant inhibition on LPA induced cell migration(P0.05,compared with control group).Conclusion:The results indicate that activation of PKG Ⅱ can inhibit migration of human gastric cancer cells.
出处
《江苏大学学报(医学版)》
CAS
2010年第2期113-116,共4页
Journal of Jiangsu University:Medicine Edition
基金
江苏省自然科学基金资助项目(BK2007091)
江苏大学博士创新基金资助项目(08JDG033)