改良型Ogu-NWSUAF CMS油菜的生物学特征

Characteristics of an improved Ogu-NWSUAF CMS in Brassica Napus L.

【目的】分析细胞质雄性不育油菜Ogu-NWSUAF CMS与Ogu CMS油菜不育系的异同,并筛选其恢复系。【方法】比较了苗期、花期、成熟期Ogu-NWSUAF CMS和Ogu CMS油菜植株形态上的差异;以Ogu-NW-SUAF CMS为母本,以国内外130份油菜品种(系)为父本,测定Ogu-NWSUAF CMS与Ogu CMS油菜恢保关系的异同;采用石蜡切片法从细胞水平上研究二者花药败育时期的特征;利用位点特异PCR法,研究二者在分子水平上的差异。【结果】Ogu-NWSUAF CMS油菜叶色正常、蜜腺发育良好、雄蕊败育彻底、天然结实性好,其综合性状优于Ogu CMS油菜;Ogu-NWSUAF CMS和Ogu CMS油菜的恢保关系不同,鉴定出了2份Ogu-NWSUAF CMS油菜的恢复系;Ogu-NWSUAF CMS油菜花药发育受不育基因的影响较晚,能分化出4个花粉囊,小孢子败育于单核期;在Ogu-NWSUAF CMS油菜中能同时扩增出Ogu系统和Nap系统的特征片段。【结论】Ogu-NWSUAF CMS油菜克服了Ogu CMS油菜的黄化缺绿现象,雄蕊败育彻底,天然结实性更好,与Ogu CMS油菜的恢保关系不同,二者在细胞和分子水平上均存在差异。

【Objective】 This study was carried out to reveal the differences between the Ogu-NWSUAF CMS line and the Ogu CMS line in Brassica napus L.,and to screen out the restorers for Ogu-NWSUAF CMS.【Method】 We compared morphological differences between the Ogu-NWSUAF CMS line and the Ogu CMS line in seedling,flowering and maturity stage.Restoring and maintaining relationship of the Ogu-NWSUAF CMS line and the Ogu CMS line was tested by 130 rapeseed accessions.Anther abortion of both lines was observed by paraffin section method.Molecular characteristics of both lines was revealed by sitespecific PCR method.【Result】 The Ogu-NWSUAF CMS line restored etiolation of the Ogu CMS line,had normal nectary,and its stamen still aborted thoroughly.The restoring and maintaining relationship of the Ogu-NWSUAF CMS line and the Ogu CMS line was different,two restorers for the Ogu-NWSUAF CMS line were identified.The Ogu-NWSUAF CMS line could form four microdiodanges.Pollen abortion of the Ogu-NWSUAF CMS line belonged to the uninucleate abortion type.Molecular characteristics by genespecific PCR showed that the Ogu-NWSUAF CMS line could amplify both Ogu CMS system specific geneorf138 product and Nap CMS system specific geneorf 222 product.【Conclusion】 The Ogu-NWSUAF CMS line restored etiolation of the Ogu CMS line,and its stamen still aborted thoroughly.The Ogu-NWSUAF CMS line had different restoring and maintaining relationship with the Ogu CMS line.There existed some differences between them at the cellular and molecular level.