摘要
【目的】对脱酚棉籽蛋白限制性酶解因素进行分析,为提高棉籽蛋白的溶解性提供参考。【方法】采用胰蛋白酶对脱酚棉籽蛋白(CPF)进行限制性酶解,以蛋白质的溶解度和水解度为指标,研究浸泡时间、搅拌方式以及酶解因素(酶用量、体系pH、底物(CPF)质量浓度、酶解温度、时间)对棉籽蛋白水解的影响。【结果】将棉籽蛋白于酶解前在3倍量(mL/g)去离子水中浸泡3h有利于酶解。在酶用量为75kU/g、pH为8、底物质量浓度为80g/L、50℃条件下磁力搅拌(120r/min)酶解3h,可得到水解度为10.67%、溶解度为54.47%的酶解棉籽蛋白。溶解度分析结果表明,限制性酶解棉籽蛋白在pH1~11时具有较好的溶解度,且保持了棉籽蛋白酸溶性的特点。【结论】用胰蛋白酶限制性水解脱酚棉籽蛋白,既可以显著提高棉籽蛋白的溶解度,又保持了其酸溶性的特点。
[Objective] To provide some references for improving the solubility of cottonseed protein, the limited enzymolysis parameters of de-gossypol cottonseed protein were analyzed. [Method] The cottonseed protein was partially hydrolyzed by trypsinase. Taking solubility and degree of hydrolysis (DH) as e- valuation index,the steeping time before enzymolysis,mixing ways and the enzymolysis parameters (such as trypsinase concentration, substrate concentration, pH value and temperature) were investigated in this paper. [Result] Steeping the cottonseed protein flour in three times de-ionized water (mL/g) before en- zyme hydrolysis is helpful to enzymolysis. In this test, a proper DH (10. 67%) and a higher solubility (54.47%) of cottonseed protein can be obtained on the condition of trypsinase concentration 75 kU/g,pH value 8,CPF mass concentration 80 g/L,the temperature 50℃ and hydrolysed 3 h with magnetic force stiring (120 r/min). The results of solubilty analyzed in different pH solutions show that the enzymolytic cottonseed protein has higher solubility in pH value from 1 to 11 ,and it still maintains the acid-soluble character of cottonseed protein before enzymolysis. [Conclusion] The solubility of de-gossypol cottonseed protein can be significantly improved by limited hydrolyzed with trypsinase in proper condition,and the acid-soluble character of cottonseed protein is remained.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2010年第4期195-200,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
安徽高校省级自然科学研究项目(KJ2009B146)
安徽省应用化学省级重点学科建设项目(No.200802187C)
滁州学院博士科研启动基金(2007-12)
关键词
胰蛋白酶
限制性酶解
棉籽蛋白
水解度
溶解度
trypsinase
limited enzymolysis
cottonseed protein
degree of hydrolysis
solubility