小麦胚芽蛋白小分子酶解产物清除自由基能力研究

Study on elimination to free radical of wheat germ protein small molecular hydrolysates

利用SephadexG–15凝胶过滤层析和DEAE52纤维素离子交换层析对小麦胚芽蛋白酶解产物进行纯化,获得平均分子量为850Da小分子活性肽,并采用Fenton反应、邻苯三酚自氧化法和DPPH法研究小分子活性肽清除自由基能力。结果表明:此类小分子活性肽对超氧阴离子、羟自由基、DPPH·具有较好清除作用,均呈一定量效关系;且清除O2–·能力与谷胱甘肽(GSH)相当。

Further purification of weat germ protein hydrolysates by using the SephadexG-15 gel filtration chromatographic and DEAE52 cellulose ionic exchange chromatographic analysis, Obtained the small active peptide which mean molecular weight is 850 Da, And studied the scavenging radical ability of small active peptide by using the Fenton reaction, the pyrogallol system and DPPH. The result indicated that： this kind of small active peptide has well scavenging action to the superoxide anion, the hydroxy radical and DPPH＂ , presents certain quantity effect relations, And scavenging ability between the O2-. and GSH in the main index.