氯乙烯影响雄性大鼠睾酮水平研究

Study of Vinyl Chloride on Testosterone Levels of Male Rats

目的探讨氯乙烯对雄性大鼠睾酮水平的影响和可能的机理。方法每组6只,4组雄性SD大鼠腹腔注射染毒28d,剂量分别为0、10、100和1000mg/kg.d。应用酶联免疫吸附法测定大鼠血清和睾丸组织中睾酮(testosterone,T)的水平,用荧光定量PCR测定睾丸组织中睾酮合成的限速酶P450胆固醇侧链裂解酶(P450cholesterol side-chain cleavage enzyme,P450scc)和类固醇激素合成急性调节蛋白(steroidogenetic acute regulatory protein,StAR)的mRNA水平。结果染毒28d后,相比对照组,100和1000mg/kg.d剂量组血清和睾丸中的睾酮水平明显下降,并有明显的剂量-反应关系;两者StAR mRNA表达水平显著下降;1000mg/kg.d剂量组的P450scc mRNA表达水平亦明显下降。结论氯乙烯对雄性大鼠血清和睾丸中的睾酮水平有影响,睾丸中StAR和P450scc mRNA水平的下调可能是其机制之一。

Objective To explore the impact of vinyl chloride on testosterone levels in male rats and its possible mechanisms . Methods 24 male SD rats were randomly divided into 4 groups and then administrated with vinyl chloride by intraperitoneal injection at different doses of 0, 10, 100, 1000 mg/kg · d for 28 days. Enzyme- linked immunosorbent assay （ELISA） was used to examine the testosterone levels of serum and testis. Meanwhile, fluorescent quantitation PCR was used to detect the mRNA expression levels of rate - limiting enzyme P450 cholesterol side - chain cleavage enzyme and steroidogenetic acute regulatory protein in the biosynthesis of testosterone. Results In 100 and 1000 mg/kg · d group, the testosterone levels of serum and testis were obviously declined compared with the control group, and it was found that there was remarkable dose - response relationship between dosages and testosterone levels. Similarly, the expression levels of StAR mRNA were also significantly lowered than that of control group. In addition, the expression level of P450scc in 10130 mg/kg · d group was significantly decreased in comparison with the control group. Conclusion Vinyl chloride has adverse effect on the testosterone levels of serum and testis in male rats and its possible mechanism maybe due to the downregulation of the expression levels of StAR and P450scc.