摘要
目的研究蝶酸(pterinacid,PTA)对天然蓖麻毒素(ricin)及重组蓖麻毒素A链蛋白(rRTA)的拮抗作用。方法用无细胞体系中荧光素酶合成抑制实验和体外细胞毒性实验评价PTA对全分子ricin和rRTA的拮抗效果。结果PTA在两种体系中均显示出了对ricin和rRTA的拮抗作用,且呈剂量依赖性。结论利用PTA作为小分子探针,建立了针对ricin/RTA小分子抑制剂的体外生物学评价系统,为以后用于拮抗ricin/RTA的全新小分子化合物筛选奠定了基础。
Objective To study the inhibitory effect of pterin acid （PTA） against ricin and recombinant ricin A chain protein.Methods Luciferase protein synthesis inhibition assay in a cell-free system and in vitro cytotoxicity experiments were performed to assess the biological activity of ricin and rRTA treated with PTA.Results The result showed that PTA could significantly inhibit the activity of ricin and rRTA in a dose-dependent manner.Conclusion PTA might be used as a small molecular probe to develop an evaluating system for ricin/RTA small molecular inhibitor in vitro.The cell-free system adopted in the current study could also serve as a necessary basis for screening some novel small molecular compounds against ricin and RTA in the future.
出处
《军事医学科学院院刊》
CSCD
北大核心
2010年第1期12-15,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家"863"计划重大项目(2006AA02A254)
