高住高练和高住低练对大鼠血脂及腓肠肌脂肪酸氧化的影响

Effect of HiHi and HiLo on the Lipid Metabolism in Rats

目的:从血脂和脂肪酸氧化的角度研究高住高练和高住低练对大鼠脂代谢的影响。方法:经适应性训练筛选30只雄性SD大鼠,采用双盲法分成低住低练组(LoLo)、高住高练组(HiHi)和高住低练组(HiLo)。采用水平动物跑台进行耐力训练,运动强度为常氧下35m/min、低氧下30m/min,1h/d,6d/周,持续训练4周。最后一次训练后恢复24h取血和腓肠肌。全自动生化分析仪检测血脂4项,ELISA法检测血清脂蛋白酯酶(LPL)、瘦素(Leptin)、脂联素(AD),荧光定量PCR测试腓肠肌PPARα和CPT-1 mRNA表达。结果:高住高练组大鼠血清TC和TG水平较低住低练组显著下降(P<0.05),LPL、AD水平显著升高(P<0.01);腓肠肌PPARαmRNA和CPT-1 mRNA表达均显著升高(P<0.05),其它指标无显著性差异。高住低练组大鼠血清HDL较低住低练组显著降低(P<0.05);腓肠肌CPT-1 mRNA相对表达量显著降低(P<0.01);其余指标无显著性变化。高住高练组大鼠血清HDL较高住低练组显著升高(P<0.05);LPL、AD水平和腓肠肌CPT-1 mRNA显著升高(P<0.01),其余指标无显著性差异。结论:(1)高住高练调节血脂变化的作用优于常氧训练,可能与提高血清LPL水平有关,高住高练较常氧训练更能促进腓肠肌脂肪酸氧化,可能与提高血清AD和腓肠肌PPARαmRNA、CPT-1 mRNA表达关系密切;(2)高住低练较常氧训练对血脂代谢无有利影响,对腓肠肌脂肪酸氧化起抑制作用;(3)高住高练对血清HDL影响优于高住低练,可能与提高血清LPL有关,高住高练较高住低练更能促进腓肠肌脂肪酸氧化,可能与提高血清AD和腓肠肌CPT-1mRNA表达关系密切。

Aim To study the effects of HiHi and HiLo on the lipid metabolism in rats through blood lipids and fatty acid oxidation in gastrocnemius of rats.Methods Thirty male SD rats were selected by means of adaptive training,and then divided into 3 groups randomly： living low-training low group（LoLo）,living high-training high group（HiHi）,and living high-training low group（HiLo）.The rats ran on a treadmill 60 min a day at the speed of 35 m/min under normoxic condition or 30 m/min under hypoxic condition（13.6% of ambient FIO2）,6 days a week for 4 weeks.The samples from blood and gastrocnemius were removed 24 hours after the last training by the end of 4-week experiment.TC,TG,HDL and LDL were tested with a full-automatic biochemical analyzer.LPL,leptin and AD were measured using ELISA.Real-time quantitative PCR was adopted to test the expression of PPARα and CPT-1 mRNA in rats’gastrocnemius.Results Compared to the LoLo,TC and TG decreased significantly（P 〈0.05）and LPL and AD increased significantly（P〈0.01）in HiHi.PPARα mRNA and CPT-1 mRNA expressions in gastrocnemius were significantly higher in HiHi（P 〈0.05）than in LoLo,and significant descent of HDL（P 〈0.05）and CPT-1 mRNA expression（P〈 0.01）revealed in HiLo,as compared to the LoLo.Compared to the HiLo,HDL（P〈 0.05）,and LPL,AD and CPT-1 mRNA expression（P 0.01）enhanced in HiHi.Conclusion（1）HiHi was more superior in regulation of blood lipids than LoLo due to increasing of LPL,and could facilitate fatty acid oxidation because of enhancement of CPT-1 mRNA,AD and PPARα mRNA.（2）Comparing to the normoxic training,HiLo had no beneficial effect on the blood lipid metabolism and decreased the fatty acid oxidation in gastrocnemius.（3）HiHi was more superior in the effeet of HDL thah HiLo due to increasing of LPL,and could facilitate fatty acid oxidation because of enhancement of AD and CPT-1 mRNA.