地尔硫卓对刀豆凝集素诱导单个核细胞细胞因子表达的影响

Effect of diltiazem on cytokines expression in mononuclearcells induced by concanavalin A

目的探讨地尔硫卓对刀豆凝集素(ConA)诱导单个核细胞细胞因子表达的影响。方法ficoll密度梯度离心法分离大鼠脾脏单个核细胞,分对照组、ConA组和地尔硫卓-ConA处理组。ELISA测定上清中细胞因子浓度。结果单个核细胞在ConA的刺激下,其上清液中IL-10、TNF-α、IL-6急剧增高,IL-1β低水平表达,TGF-β1未见明显表达。地尔硫卓干预后,IL-10、TNF-α、IL-6的浓度明显减低。结论地尔硫卓能抑制ConA所诱导的单个核细胞IL-10、TNF-α、IL-6的表达。

Aim To research into the effect of diltiazem on cytokines expression in mononuclearcells induced by concanavalin A.Methods Ficoll density gradient centrifugation was used to separate the mononuclearcells from rat＇s spleen.There were 3 groups including control,Con A,diltiazem-Con A group in the study.The cytokines expressions in supernatant were detected by ELISA.Results Compared with control,IL-10,TNF-α,IL-6 were increased significantly in Con A group with low level IL-1β and non level TGF-β1.But in diltiazem-Con A group,IL-10,TNF-α,IL-6 were decreased significantly compared with Con A group.Conclusion Diltiazem inhibits IL-10,TNF-α,IL-6 expressions in mononuclearcells induced by Con A.