食管癌贲门癌患者T淋巴细胞、NK细胞免疫功能检测及意义

Detecting of T lymph cells and NK cells of esophageal carcinoma and cardia carcinoma patients and their significance

目的本研究检测食管癌、贲门癌患者的免疫功能,寻找肿瘤患者的病情量化指标,用来预测和判定临床治疗效果。方法采集本院胸外科手术前的食管癌贲门癌患者115例及359例正常体检者的血液标本,应用流式细胞术检测外周血T淋巴细胞表面CD3、CD4、CD8及NK细胞(CD56)的表达。结果食管癌、贲门癌组与健康对照组相比较CD3、CD4有明显下降(P<0.05),而CD8、CD56有明显的增高(P<0.05)。将癌症组分为淋巴结转移组和无淋巴转移组,两组间CD3、CD4、CD8、CD56的差异均无统计学意义(P>0.05)。结论食管癌外周血T细胞免疫检测有助于了解疾病的发展进程。

Objective To detect the immunity function of esophageal carcinoma and cardia carcinoma patients,find the state of patients quantification index,and predict and judge clinical therapeutic effect.Methods Blood samples from 115 cases of esophageal carcinoma and cardia carcinoma patients and 359 normal people were collected.The expressions of CD3,CD4,CD8 and NK cells （CD56） on the surface of T lymphocytes were examined by flow cytometer.Results The expressions of CD3,CD4,CD8 and CD56 in esophageal carcinoma were 63.073±11.241,30.370±11.549,36.242±11.449 and 17.667±10.532 respectively.The expressions of CD3,CD4,CD8,and CD56 in normal people were 71.252±10.532,39.489±10.939,29.179±9.894 and 14.333±8.345 respectively.The expressions of CD3 and CD4 in esophageal carcinoma and cardia carcinoma group were decreased compared with those in normal people significantly （P0.05）,while the expressions of CD8 and CD56 were increased （P0.05）.Carcinoma group was divided into lymphatic metastasis and non-lymphatic metastasis groups.There was no difference between the lymphatic metastasis and non-lymphatic metastasis groups in the expressions of CD3,CD4,CD8 and CD56.Conclusions Detecting the T cell immunity function of esophageal carcinoma is beneficial to find out the development process of disease.