Ghrelin对高糖诱导心肌细胞凋亡的影响

The effects of Ghrelin on hyperglycemia-induced apoptosis in cultured cardiomyocyte

目的探讨Ghrelin对高糖诱导心肌细胞凋亡的影响。方法将原代培养的心肌细胞随机分为:①正常对照组(N组);②高糖组(H组);③高糖+Ghrelin组(G组)。应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测各组心肌细胞凋亡,分光光度法检测心肌细胞内caspase-3活性的变化。结果与N组比较,H组心肌细胞凋亡明显增加,caspase-3活性显著升高;与H组比较,G组心肌细胞凋亡数量明显减少,caspase-3活性明显降低(均P<0.05)。结论Ghrelin可能通过降低caspase-3活性抑制高糖诱导的心肌细胞凋亡。

Objective To investigate the effects of Ghrelin on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte.Methods The cultured cardiomyocytes were randomly divided into Group N （cardiomyocytes exposed to low glucose level,5.5 mmol/L）;Group H （cardiomyocytes were cultured with high glocose level,25.0 mmol/L）;Group G（cardiomyocytes were cultured in both high glucose level,25.0 mmol/L and Ghrelin）.Apoptosis in different groups was determined by TUNEL.The change of caspase-3 activity in cardiomyocytes was detected by caspase-3 activity assay kit.Results Compared with that of group N,the number of apoptosis cardiomyocytes was significantly increased in group H.The caspase-3 activity in group H was higher than that in group N.The number of apoptosis cardiomyocytes was significantly decreased in group G relative to group H,and the caspase-3 activity in group G was markedly lower than that in group H.（P0.05）.Conclusions Ghrelin could effectively inhibit hyperglycemia-induced apoptosis in cultured rat cardiomyocyte by decreasing caspase-3 activity.